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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using CD100 Recombinant antibody (84465-4-RR)

    IF Staining of Jurkat using 84465-4-RR

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CD100 antibody (84465-4-RR, Clone: 241708D10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84465-4-RR

    Affinity and Kinetic Characterization of 84465-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84465-4-RR against Human CD100 were performed. The affinity constant is 3.21 nM.

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CD100 Recombinant antibody

CD100 Recombinant Antibody for IF/ICC, ELISA
Cat No. 84465-4-RR
Clone No.241708D10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Synonyms

SEMA4D, SEM4D, CD100 antigen, CD 100, A8

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive IF/ICC detected inJurkat cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84465-4-RR targets CD100 in IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant Protein 相同性解析による交差性が予測される生物種
Full Name sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D
Calculated molecular weight 96 kDa
GenBank accession numberBC054500
Gene Symbol SEMA4D/CD100
Gene ID (NCBI) 10507
RRIDAB_3671987
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ92854
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD100 (also known as SEMA4D), a 150 kDa homodimer, belongs to the family of immune semaphorins, and CD100 is abundantly expressed on resting T cells, NK cells, and antigen-presenting cells. As a transmembrane glycoprotein, it is digested to its soluble form, sCD100, by specific matrix metalloproteinases. CD100 has been reported that the expression levels of CD100, and one of its receptors, plexin-B1 (PLXNB1), are increased in head and neck, prostate, colon, breast, and lung cancers, and the interaction between them provides oncogenic signaling essential for tumor growth and metastasis.

Protocols

Product Specific Protocols
IF protocol for CD100 antibody 84465-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols