Validation Data Gallery

Filter: AllFC (Intra)

FC experiment of HeLa using 65519-1-MR (same clone as 65519-1-PBS)
1x10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human CD107a Mouse Recombinant Antibody (65519-1-MR, Clone:H4A3) or Mouse IgG1 Isotype Control (65124-1-Ig, Clone: MOPC-21) and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Mouse IgG (H+L) (SA00014-8). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 65519-1-PBS in a different storage buffer formulation.
×
1x10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human CD107a Mouse Recombinant Antibody (65519-1-MR, Clone:H4A3) or Mouse IgG1 Isotype Control (65124-1-Ig, Clone: MOPC-21) and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Mouse IgG (H+L) (SA00014-8). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 65519-1-PBS in a different storage buffer formulation.
FC experiment of HeLa using 65519-1-PBS
1x10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human CD107a Mouse Recombinant Antibody (65519-1-PBS, Clone:H4A3) or Mouse IgG1 Isotype Control (65124-1-Ig, Clone: MOPC-21) and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Mouse IgG (H+L) (SA00014-8). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
FC experiment of HeLa using 65519-1-PBS
1x10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human CD107a Mouse Recombinant Antibody (65519-1-PBS, Clone:H4A3) or Mouse IgG1 Isotype Control (65124-1-Ig, Clone: MOPC-21) and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Mouse IgG (H+L) (SA00014-8). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
FC experiment of HeLa using 65519-1-MR (same clone as 65519-1-PBS)
1x10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human CD107a Mouse Recombinant Antibody (65519-1-PBS, Clone:H4A3) or Mouse IgG1 Isotype Control (65124-1-Ig, Clone: MOPC-21) and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Mouse IgG (H+L) (SA00014-8). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 65519-1-PBS in a different storage buffer formulation.
×
1x10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human CD107a Mouse Recombinant Antibody (65519-1-PBS, Clone:H4A3) or Mouse IgG1 Isotype Control (65124-1-Ig, Clone: MOPC-21) and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Mouse IgG (H+L) (SA00014-8). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 65519-1-PBS in a different storage buffer formulation.
FC experiment of HeLa using 65519-1-MR (same clone as 65519-1-PBS)
1x10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human CD107a Mouse Recombinant Antibody (65519-1-PBS, Clone:H4A3) (left and red) or 0.2 ug Anti-Human CD107a Mouse Monoclonal Antibody (65051-1-Ig, Clone:H4A3) (center and black dashed) or Mouse IgG1 Isotype Control (65124-1-Ig, Clone: MOPC-21) (blue) and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Mouse IgG (H+L) (SA00014-8). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 65519-1-PBS in a different storage buffer formulation.
×
1x10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human CD107a Mouse Recombinant Antibody (65519-1-PBS, Clone:H4A3) (left and red) or 0.2 ug Anti-Human CD107a Mouse Monoclonal Antibody (65051-1-Ig, Clone:H4A3) (center and black dashed) or Mouse IgG1 Isotype Control (65124-1-Ig, Clone: MOPC-21) (blue) and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Mouse IgG (H+L) (SA00014-8). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 65519-1-PBS in a different storage buffer formulation.
FC experiment of HeLa using 65519-1-PBS
1x10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human CD107a Mouse Recombinant Antibody (65519-1-PBS, Clone:H4A3) (left and red) or 0.2 ug Anti-Human CD107a Mouse Monoclonal Antibody (65051-1-Ig, Clone:H4A3) (center and black dashed) or Mouse IgG1 Isotype Control (65124-1-Ig, Clone: MOPC-21) (blue) and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Mouse IgG (H+L) (SA00014-8). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
×
1x10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human CD107a Mouse Recombinant Antibody (65519-1-PBS, Clone:H4A3) (left and red) or 0.2 ug Anti-Human CD107a Mouse Monoclonal Antibody (65051-1-Ig, Clone:H4A3) (center and black dashed) or Mouse IgG1 Isotype Control (65124-1-Ig, Clone: MOPC-21) (blue) and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Mouse IgG (H+L) (SA00014-8). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Anti-Human CD107a / LAMP1 (H4A3) Mouse IgG1 Recombinant Antibody, PBS Only

CD107a / LAMP1 Recombinant Antibody for FC (Intra)

Cat No. 65519-1-PBS

Clone No.H4A3

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

FC (Intra)

Synonyms

CD107a, LAMP1, LAMP-1, LAMP 1, H4A3

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive FC (Intra) detected in

HeLa cells

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

65519-1-PBS targets CD107a / LAMP1 in FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Mouse / IgG1
Class	Recombinant
Type	Antibody
Immunogen	Human adherent peripheral blood cells 相同性解析による交差性が予測される生物種
Full Name	lysosomal-associated membrane protein 1
Calculated molecular weight	45 kDa
GenBank accession number	BC006345
Gene Symbol	LAMP1
Gene ID (NCBI)	3916
ENSEMBL Gene ID	ENSG00000185896
Conjugate	Unconjugated
Form	Liquid
Purification Method	Affinity purification
Storage Buffer	PBS only
Storage Conditions	Store at -80°C. Stable for one year after shipment.

Background Information

LAMP1 (CD107a) is a heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP1 is expressed largely in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane (PMID: 16168398). Elevated LAMP1 expression at the cell surface has also been detected during platelet and granulocytic cell activation, as well as in some tumor cells (PMID: 29085473). LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis (PMID: 18835598; 29085473; 9426697).

Protocols

Product Specific Protocols
FC protocol for CD107a / LAMP1 antibody 65519-1-PBS	Download protocol

Standard Protocols
Click here to view our Standard Protocols

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Validation Data Gallery

FC experiment of HeLa using 65519-1-MR (same clone as 65519-1-PBS)

FC experiment of HeLa using 65519-1-PBS

FC experiment of HeLa using 65519-1-PBS

FC experiment of HeLa using 65519-1-MR (same clone as 65519-1-PBS)

FC experiment of HeLa using 65519-1-MR (same clone as 65519-1-PBS)

FC experiment of HeLa using 65519-1-PBS

Anti-Human CD107a / LAMP1 (H4A3) Mouse IgG1 Recombinant Antibody, PBS Only

CD107a / LAMP1 Recombinant Antibody for FC (Intra)

-/ -

Tested Applications

Recommended dilution

Product Information

Background Information

Protocols