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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Anti-Human CD107a / LAMP1 (H4A3) (65051-1-Ig)

    WB analysis using 65051-1-Ig (same clone as 65051-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 65051-1-Ig (CD107a / LAMP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 65051-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Anti-Human CD107a / LAMP1 (H4A3) (65051-1-Ig)

    IF Staining of HeLa using 65051-1-Ig (same clone as 65051-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 65051-1-Ig (CD107a antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 65051-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Anti-Human CD107a / LAMP1 (H4A3) (65051-1-Ig)

    IF Staining of HeLa using 65051-1-Ig (same clone as 65051-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using 65051-1-Ig (CD107a antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 65051-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using Anti-Human CD107a / LAMP1 (H4A3) (65051-1-Ig)

    FC experiment of HeLa using 65051-1-Ig (same clone as 65051-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human CD107a / LAMP1 (65051-1-Ig, Clone:H4A3) and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 65051-1-PBS in a different storage buffer formulation.

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Anti-Human CD107a / LAMP1 (H4A3), PBS Only

CD107a / LAMP1 Monoclonal Antibody for WB, IF/ICC, FC (Intra)
Cat No. 65051-1-PBS
Clone No.H4A3

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human

Applications

WB, IF/ICC, FC (Intra)

Synonyms

CD107a, LAMP1, LAMP-1, LAMP 1, H4A3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

65051-1-PBS targets CD107a / LAMP1 in WB, IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen Human adherent peripheral blood cells 相同性解析による交差性が予測される生物種
Full Name lysosomal-associated membrane protein 1
Calculated molecular weight45 kDa
Observed molecular weight100-120 kDa
GenBank accession numberBC006345
Gene Symbol LAMP1
Gene ID (NCBI) 3916
ENSEMBL Gene IDENSG00000185896
RRIDAB_2881467
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP11279
Storage Buffer PBS only
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

LAMP1 (CD107a) is a heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP1 is expressed largely in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane (PMID: 16168398). Elevated LAMP1 expression at the cell surface has also been detected during platelet and granulocytic cell activation, as well as in some tumor cells (PMID: 29085473). LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis (PMID: 18835598; 29085473; 9426697).