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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human CD107b / LAMP2 (H4B4) Rabbit IgG Recombinant Antibody, PBS Only

CD107b / LAMP2 Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

FC (Intra)

Conjugate

Unconjugated

CloneNo.

H4B4

Cat no : 65520-1-PBS

Synonyms

LAMP2, CD107b, H4B4, LAMP 2, CD107 antigen-like family member B, LAMP-2, LAMPB, LGP110, LGP-96, Lysosome-associated membrane glycoprotein 2, Lysosome-associated membrane protein 2

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD107b (H4B4) (65520-1-PBS)

    FC experiment of human PBMCs using 65520-1-PBS

    1x10^6 human PBMCs were intracellularly stained with 0.2 ug Anti-Human CD107b Rabbit Recombinant Antibody (65520-1-PBS, Clone:H4B4) or Rabbit IgG control Rabbit PolyAb, and CoraLite®647-Conjugated Donkey Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Cells were treated with FC Receptor Block prior to staining.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD107b (H4B4) (65520-1-PBS)

    FC experiment of human PBMCs using 65520-1-PBS

    1x10^6 human PBMCs were intracellularly stained with 0.2 ug Anti-Human CD107b Rabbit Recombinant Antibody (65520-1-PBS, Clone:H4B4) or Rabbit IgG control Rabbit PolyAb, and CoraLite®647-Conjugated Donkey Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Cells were treated with FC Receptor Block prior to staining.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD107b (H4B4) (65520-1-PBS)

    FC experiment of human PBMCs using 65520-1-PBS

    1x10^6 human PBMCs were intracellularly stained with 0.2 ug Anti-Human CD107b Rabbit Recombinant Antibody (65520-1-PBS, Clone:H4B4) and CoraLite®647-Conjugated Donkey Anti-Rabbit IgG(H+L) at dilution 1:1000 (left) or 0.2 ug Anti-Human CD107b Mouse Monoclonal Antibody (Clone:H4B4) and CoraLite®647-Conjugated Donkey Anti-Mouse IgG(H+L) at dilution 1:1000 (center and right). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Cells were treated with FC Receptor Block prior to staining.

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Tested Applications

Positive FC (Intra) detected inhuman PBMCs
Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

65520-1-PBS targets CD107b / LAMP2 in FC (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Human adherent peripheral blood cells 相同性解析による交差性が予測される生物種
Full Name lysosomal-associated membrane protein 2
Calculated molecular weight 45 kDa
GenBank accession numberBC002965
Gene symbol LAMP2
Gene ID (NCBI) 3920
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C. Stable for one year after shipment.

Background Information

LAMP2 (CD107b) is a Lysosomal membrane glycoprotein. LAMP2 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP-2 is localized primarily in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane under certain circumstances, e.g., after platelet activation, during granulocytic differentiation and activation, and in some tumor cells (PMID: 12221139). LAMP is involved in lysosomal stability and autophagy (PMID: 12221139). This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may play a role in tumor cell metastasis (PMID 9426697).

Protocols

Product Specific Protocols
FC protocol for CD107b / LAMP2 antibody 65520-1-PBSDownload protocol
Standard Protocols
Click here to view our Standard Protocols