CoraLite®555 Anti-Mouse CD107b / LAMP2 (ABL-93)
CD107b Monoclonal Antibody for IF
Host / Isotype
Rat / IgG2a
Reactivity
Mouse
Applications
IF
Conjugate
CoraLite®555 Fluorescent Dye
CloneNo.
ABL-93
Cat no : CL555-65052
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF/ICC detected in | NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL555-65052 targets CD107b in IF applications and shows reactivity with Mouse samples.
| Tested Reactivity | Mouse |
| Host / Isotype | Rat / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Glycoproteins purified from BALB/c mouse embryo 3T3 cell line 相同性解析による交差性が予測される生物種 |
| Full Name | lysosomal-associated membrane protein 2 |
| GenBank accession number | BC138718 |
| Gene symbol | CD107b / LAMP2 |
| Gene ID (NCBI) | 16784 |
| Conjugate | CoraLite®555 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 557 nm / 570nm |
| Form | Liquid |
| Purification Method | N/A |
| Storage Buffer | PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
LAMP2 (CD107b) is a Lysosomal membrane glycoprotein. LAMP2 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP-2 is localized primarily in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane under certain circumstances, e.g., after platelet activation, during granulocytic differentiation and activation, and in some tumor cells (PMID: 12221139). LAMP is involved in lysosomal stability and autophagy (PMID: 12221139). This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may plays a role in tumor cell metastasis (PMID 9426697).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL555 CD107b antibody CL555-65052 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
