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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 647 Anti-Human CD107b / LAMP2 (H4B4) Rabbit IgG Recombinant Antibody

CD107b / LAMP2 Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

H4B4

Cat no : CL647-65520

Synonyms

CD107b, LAMP 2, LAMP2, LAMPB, LGP110

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using CoraLite® Plus 647 Anti-Human CD107b (H4B4) Rabbit (CL647-65520)

    FC experiment of human PBMCs using CL647-65520

    1x10^6 human PBMCs were intracellularly stained with 5 ul CoraLite® Plus 647 Anti-Human CD107b Rabbit Recombinant Antibody (CL647-65520, Clone:H4B4) or CoraLite® Plus 647-conjugated Rabbit IgG control PolyAb (CL647-30000). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Cells were treated with FC Receptor Block prior to staining.

  • Flow cytometry (FC) experiment of human PBMCs using CoraLite® Plus 647 Anti-Human CD107b (H4B4) Rabbit (CL647-65520)

    FC experiment of human PBMCs using CL647-65520

    1x10^6 human PBMCs were intracellularly stained with 5 ul CoraLite® Plus 647 Anti-Human CD107b Rabbit Recombinant Antibody (CL647-65520, Clone:H4B4) or CoraLite® Plus 647-conjugated Rabbit IgG control PolyAb (CL647-30000). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Cells were treated with FC Receptor Block prior to staining.

  • Flow cytometry (FC) experiment of human PBMCs using CoraLite® Plus 647 Anti-Human CD107b (H4B4) Rabbit (CL647-65520)

    FC experiment of human PBMCs using CL647-65520

    1x10^6 human PBMCs were intracellularly stained with 5 ul CoraLite® Plus 647 Anti-Human CD107b Rabbit Recombinant Antibody (CL647-65520, Clone:H4B4) or 5 ul CoraLite® Plus 647 Anti-Human CD107b Mouse Monoclonal Antibody (CL647-65053, Clone:H4B4). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Cells were treated with FC Receptor Block prior to staining.

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Tested Applications

Positive FC (Intra) detected inhuman PBMCs
Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-65520 targets CD107b / LAMP2 in FC (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Human adherent peripheral blood cells 相同性解析による交差性が予測される生物種
Full Name lysosomal-associated membrane protein 2
Calculated molecular weight 45 kDa
GenBank accession numberBC002965
Gene symbol LAMP2
Gene ID (NCBI) 3920
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

LAMP2 (CD107b) is a Lysosomal membrane glycoprotein. LAMP2 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP-2 is localized primarily in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane under certain circumstances, e.g., after platelet activation, during granulocytic differentiation and activation, and in some tumor cells (PMID: 12221139). LAMP is involved in lysosomal stability and autophagy (PMID: 12221139). This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may play a role in tumor cell metastasis (PMID 9426697).

Protocols

Product Specific Protocols
FC protocol for CL Plus 647 CD107b / LAMP2 antibody CL647-65520Download protocol
Standard Protocols
Click here to view our Standard Protocols