CoraLite® Plus 647 Anti-Human CD107b / LAMP2 (H4B4) Rabbit IgG Recombinant Antibody
CD107b / LAMP2 Recombinant Antibody for FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
Human
Applications
FC (Intra)
Conjugate
CoraLite® Plus 647 Fluorescent Dye
CloneNo.
H4B4
Cat no : CL647-65520
Synonyms
Validation Data Gallery
Tested Applications
Positive FC (Intra) detected in | human PBMCs |
Positive FC detected in | human PBMCs |
Recommended dilution
Application | Dilution |
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This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL647-65520 targets CD107b / LAMP2 in FC (Intra) applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | Human adherent peripheral blood cells 相同性解析による交差性が予測される生物種 |
Full Name | lysosomal-associated membrane protein 2 |
Calculated molecular weight | 45 kDa |
GenBank accession number | BC002965 |
Gene symbol | LAMP2 |
Gene ID (NCBI) | 3920 |
Conjugate | CoraLite® Plus 647 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 654 nm / 674 nm |
Form | Liquid |
Purification Method | Affinity purification |
Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA. |
Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
LAMP2 (CD107b) is a Lysosomal membrane glycoprotein. LAMP2 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP-2 is localized primarily in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane under certain circumstances, e.g., after platelet activation, during granulocytic differentiation and activation, and in some tumor cells (PMID: 12221139). LAMP is involved in lysosomal stability and autophagy (PMID: 12221139). This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may play a role in tumor cell metastasis (PMID 9426697).
Protocols
Product Specific Protocols | |
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FC protocol for CL Plus 647 CD107b / LAMP2 antibody CL647-65520 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |