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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human CD107b / LAMP2 (H4B4) Mouse IgG2a Recombinant Antibody, PBS Only

CD107b / LAMP2 Recombinant Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC (Intra)

Conjugate

Unconjugated

CloneNo.

H4B4

Cat no : 65566-1-PBS

Synonyms

CD107b, LAMP2, LAMP-2, LAMP 2, H4B4

Validation Data Gallery

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  • Flow cytometry (FC) experiment of Jurkat cells using Anti-Human CD107b / LAMP2 (H4B4) Mouse IgG2a Recom (65566-1-MR)

    FC experiment of Jurkat using 65566-1-MR (same clone as 65566-1-PBS)

    1x10^6 Jurkat cells were intracellularly stained with 0.5 ug Anti-Human CD107b / LAMP2 (H4B4) Mouse IgG2a Recombinant Antibody (65566-1-MR, Clone: H4B4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1) (red), or 0.5 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 65566-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of Jurkat cells using Anti-Human CD107b / LAMP2 (H4B4) Mouse IgG2a Recom (65566-1-PBS)

    FC experiment of Jurkat using 65566-1-PBS

    1x10^6 Jurkat cells were intracellularly stained with 0.5 ug Anti-Human CD107b / LAMP2 (H4B4) Mouse IgG2a Recombinant Antibody, PBS Only (65566-1-PBS, Clone: H4B4) and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Goat Anti-Mouse IgG (H+L) (SA00014-8) (red), or 0.5 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive FC (Intra) detected inJurkat cells

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

65566-1-PBS targets CD107b / LAMP2 in FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Immunogen Human adherent peripheral blood cells 相同性解析による交差性が予測される生物種
Full Name lysosomal-associated membrane protein 2
Calculated molecular weight 45 kDa
GenBank accession numberBC002965
Gene symbol LAMP2
Gene ID (NCBI) 3920
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C. Stable for one year after shipment.

Background Information

LAMP2 (CD107b) is a Lysosomal membrane glycoprotein. LAMP2 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP-2 is localized primarily in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane under certain circumstances, e.g., after platelet activation, during granulocytic differentiation and activation, and in some tumor cells (PMID: 12221139). LAMP is involved in lysosomal stability and autophagy (PMID: 12221139). This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may play a role in tumor cell metastasis (PMID 9426697).

Protocols

Product Specific Protocols
FC protocol for CD107b / LAMP2 antibody 65566-1-PBSDownload protocol
Standard Protocols
Click here to view our Standard Protocols