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  • Featured Product
  • KD/KO Validated

CD107b / LAMP2 Monoclonal antibody, PBS Only

CD107b / LAMP2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgA

Reactivity

human, mouse, pig

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2D3B9

Cat no : 66301-1-PBS

Synonyms

CD107b, LAMP2, LAMP-2, LAMP 2, CD107 antigen-like family member B

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD107b / LAMP2 Monoclonal antibody (66301-1-Ig)

    WB analysis using 66301-1-Ig (same clone as 66301-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66301-1-Ig (LAMP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66301-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CD107b / LAMP2 Monoclonal antibody (66301-1-Ig)

    WB analysis using 66301-1-Ig (same clone as 66301-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66301-1-Ig (LAMP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66301-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig liver tissue using CD107b / LAMP2 Monoclonal antibody (66301-1-Ig)

    WB analysis of pig liver using 66301-1-Ig (same clone as 66301-1-PBS)

    pig liver tissue were subjected to SDS PAGE followed by western blot with 66301-1-Ig (LAMP2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66301-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using CD107b / LAMP2 Monoclonal antibody (66301-1-Ig)

    WB analysis of mouse liver using 66301-1-Ig (same clone as 66301-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 66301-1-Ig (LAMP2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66301-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CD107b / LAMP2 Monoclonal antibody (66301-1-Ig)

    IHC staining of human liver cancer using 66301-1-Ig (same clone as 66301-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66301-1-Ig (LAMP2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66301-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using CD107b / LAMP2 Monoclonal antibody (66301-1-Ig)

    IHC staining of mouse liver using 66301-1-Ig (same clone as 66301-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66301-1-Ig (CD107b / LAMP2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66301-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using CD107b / LAMP2 Monoclonal antibody (66301-1-Ig)

    IHC staining of mouse liver using 66301-1-Ig (same clone as 66301-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66301-1-Ig (CD107b / LAMP2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66301-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CD107b / LAMP2 Monoclonal antibody (66301-1-Ig)

    IHC staining of human liver cancer using 66301-1-Ig (same clone as 66301-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66301-1-Ig (LAMP2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66301-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using CD107b / LAMP2 Monoclonal antibody (66301-1-Ig)

    IHC staining of human kidney using 66301-1-Ig (same clone as 66301-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66301-1-Ig (LAMP2 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66301-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using CD107b / LAMP2 Monoclonal antibody (66301-1-Ig)

    IHC staining of human kidney using 66301-1-Ig (same clone as 66301-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66301-1-Ig (LAMP2 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66301-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CD107b / LAMP2 Monoclonal antibody (66301-1-Ig)

    IHC staining of human lung cancer using 66301-1-Ig (same clone as 66301-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66301-1-Ig (LAMP2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66301-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CD107b / LAMP2 Monoclonal antibody (66301-1-Ig)

    IHC staining of human lung cancer using 66301-1-Ig (same clone as 66301-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66301-1-Ig (LAMP2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66301-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using CD107b / LAMP2 Monoclonal antibody (66301-1-Ig)

    IHC staining of human liver using 66301-1-Ig (same clone as 66301-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66301-1-Ig (LAMP2 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66301-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using CD107b / LAMP2 Monoclonal antibody (66301-1-Ig)

    IHC staining of human liver using 66301-1-Ig (same clone as 66301-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66301-1-Ig (LAMP2 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66301-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CD107b / LAMP2 Monoclonal antibody (66301-1-Ig)

    IF Staining of HeLa using 66301-1-Ig (same clone as 66301-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 66301-1-Ig (LAMP2 antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), phalloidine stains F-actin (red). This data was developed using the same antibody clone with 66301-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CD107b / LAMP2 Monoclonal antibody (66301-1-Ig)

    IF Staining of HeLa using 66301-1-Ig (same clone as 66301-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 66301-1-Ig (LAMP2 antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), phalloidine stains F-actin (red). This data was developed using the same antibody clone with 66301-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of 3T3-L1 cells using CD107b / LAMP2 Monoclonal antibody (66301-1-Ig)

    IF Staining of 3T3-L1 using 66301-1-Ig (same clone as 66301-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed 3T3-L1 cells using 66301-1-Ig (LAMP2 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66301-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CD107b / LAMP2 Monoclonal antibody (66301-1-Ig)

    IF Staining of HepG2 using 66301-1-Ig (same clone as 66301-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66301-1-Ig (LAMP2 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66301-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CD107b / LAMP2 Monoclonal antibody (66301-1-Ig)

    IF Staining of HepG2 using 66301-1-Ig (same clone as 66301-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed HepG2 cells using 66301-1-Ig(LAMP2 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66301-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66301-1-PBS targets CD107b / LAMP2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, pig samples.

Tested Reactivity human, mouse, pig
Host / Isotype Mouse / IgA
Class Monoclonal
Type Antibody
Immunogen CD107b / LAMP2 fusion protein Ag0595 相同性解析による交差性が予測される生物種
Full Name lysosomal-associated membrane protein 2
Calculated molecular weight 45 kDa
Observed molecular weight 120 kDa
GenBank accession numberBC002965
Gene symbol LAMP2
Gene ID (NCBI) 3920
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Lysosomal-associated membrane protein 2 (LAMP2, synonyms: LAMPB, CD107b) is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may play a role in tumor cell metastasis. It may also function in the protection, maintenance, and adhesion of the lysosome. Prior to posttranslational modification, LAMP2 is a ~45 kDa polypeptide. Mature, functional LAMP2 is extensively glycosylated with a variety of different N-linked and O-linked oligosaccharides.