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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Mouse CD107b / LAMP2 Recombinant antibody, PBS Only (Capture)

CD107b / LAMP2 Recombinant Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241893A7

Cat no : 84474-5-PBS

Synonyms

CD107b, Lamp2, Mac3, Lamp 2, 241893A7

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD107b / LAMP2 Recombinant antibody (84474-5-RR)

    WB analysis using 84474-5-RR (same clone as 84474-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84474-5-RR (CD107b / LAMP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84474-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using CD107b / LAMP2 Recombinant antibody (84474-5-RR)

    IHC staining of mouse liver using 84474-5-RR (same clone as 84474-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84474-5-RR (CD107b / LAMP2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84474-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using CD107b / LAMP2 Recombinant antibody (84474-5-RR)

    IHC staining of mouse liver using 84474-5-RR (same clone as 84474-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84474-5-RR (CD107b / LAMP2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84474-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using CD107b / LAMP2 Recombinant antibody (84474-5-RR)

    IF Staining of NIH/3T3 using 84474-5-RR (same clone as 84474-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using CD107b / LAMP2 antibody (84474-5-RR, Clone: 241893A7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84474-5-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01356-3

    Cytometric bead array standard curve of MP01356-3

    Cytometric bead array standard curve of MP01356-3, MOUSE LAMP-2/CD107b Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84474-5-PBS. Detection antibody: 84474-2-PBS. Standard: Eg2029. Range: 0.625-80 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84474-5-PBS targets CD107b / LAMP2 as part of a matched antibody pair:

MP01356-3: 84474-5-PBS capture and 84474-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name lysosomal-associated membrane protein 2
Calculated molecular weight46 kDa
Observed molecular weight90-110 kDa
GenBank accession numberNM_001017959.2
Gene symbol CD107b / LAMP2
Gene ID (NCBI) 16784
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

LAMP2 (CD107b) is a Lysosomal membrane glycoprotein. LAMP2 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP-2 is localized primarily in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane under certain circumstances, e.g., after platelet activation, during granulocytic differentiation and activation, and in some tumor cells (PMID: 12221139). LAMP is involved in lysosomal stability and autophagy (PMID: 12221139). This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may plays a role in tumor cell metastasis (PMID 9426697).