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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human CD11a (TS2/4) Rabbit Recombinant Antibody

CD11a Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

FC

Conjugate

Unconjugated

CloneNo.

TS2/4

Cat no : 65518-1-RR

Synonyms

CD11A, Integrin alpha L, ITGAL, LFA 1, LFA 1A, LFA1A

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD11a (TS2/4) (65518-1-RR)

    FC experiment of human PBMCs using 65518-1-RR

    1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD11a Rabbit Recombinant Antibody (65518-1-RR, Clone:TS2/4) or Rabbit IgG control Rabbit PolyAb and CoraLite®647-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD11a (TS2/4) (65518-1-RR)

    FC experiment of human PBMCs using 65518-1-RR

    1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD11a Rabbit Recombinant Antibody (65518-1-RR, Clone:TS2/4) or Rabbit IgG control Rabbit PolyAb and CoraLite®647-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD11a (TS2/4) (65518-1-RR)

    FC experiment of human PBMCs using 65518-1-RR

    1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD11a Rabbit Recombinant Antibody (65518-1-RR, Clone:TS2/4) (left) and CoraLite®647-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000, or with 0.2 ug Anti-Human CD11a Mouse Monoclonal Antibody (Clone:TS2/4) (center and right) and CoraLite®647-Conjugated Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated.

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Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65518-1-RR targets CD11a in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen HLA-DR CTL line 相同性解析による交差性が予測される生物種
Full Name integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide)
Calculated molecular weight 129 kDa
GenBank accession numberBC008777
Gene symbol CD11a
Gene ID (NCBI) 3683
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

CD11a, also known as integrin αL or LFA-1α, is a 170-180 kDa transmembrane glycoprotein that forms a heterodimer with CD18 (PMID: 7027264; 1672643). CD11a/CD18 (LFA-1) is expressed by all leukocytes and mediates cell adhesion through interactions with its ligands, intercellular adhesion molecule 1 (ICAM-1), ICAM-2, and ICAM-3 (PMID: 7479767). CD11a/CD18 also functions in lymphocyte costimulatory signaling (PMID: 1972160).

Protocols

Product Specific Protocols
FC protocol for CD11a antibody 65518-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols