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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD11b Polyclonal antibody

CD11b Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 31745-1-AP

Synonyms

Ly 40, Itgam, integrin alpha M, CR3A, CR3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD11b Polyclonal antibody (31745-1-AP)

    WB analysis using 31745-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31745-1-AP (CD11b antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inJ774A.1 cells, RAW 264.7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31745-1-AP targets CD11b in WB, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD11b fusion protein Ag36145 相同性解析による交差性が予測される生物種
Full Name integrin alpha M
Calculated molecular weight127 kDa
Observed molecular weight150 kDa
GenBank accession numberBC156991
Gene symbol CD11b
Gene ID (NCBI) 16409
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b, also known as Integrin alpha M or CR3A, belongs to the integrin alpha chain family. CD11b forms an α/β heterodimer with CD18 (integrin β2). CD11b/CD18 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PMID: 9558116; 20008295). CD11b/CD18 is a receptor for the complement protein fragment iC3b, and is also a receptor for fibrinogen, factor X and ICAM1 (PMID: 2971974; 15485828).

Protocols

Product Specific Protocols
WB protocol for CD11b antibody 31745-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols