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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Rat CD11b (OX42) Mouse IgG2a Recombinant Antibody

CD11b Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

Rat

Applications

FC

Conjugate

Unconjugated

CloneNo.

OX42

Cat no : 65606-1-MR

Synonyms

Cd11b, integrin alpha M, Itgam

Validation Data Gallery

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  • Flow cytometry (FC) experiment of rat bone marrow cells using Anti-Rat CD11b (OX42) Mouse IgG2a Recombinant Anti (65606-1-MR)

    FC experiment of rat bone marrow cells using 65606-1-MR

    1x10^6 rat bone marrow cells were surface stained with 0.25 ug Anti-Rat CD11b Mouse IgG2a Recombinant Antibody (65606-1-MR, Clone: OX42) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) and FITC anti-Mouse IgG2a Antibody. Cells were not fixed.

  • Flow cytometry (FC) experiment of rat bone marrow cells using Anti-Rat CD11b (OX42) Mouse IgG2a Recombinant Anti (65606-1-MR)

    FC experiment of rat bone marrow cells using 65606-1-MR

    1x10^6 rat bone marrow cells were surface stained with 0.25 ug Anti-Rat CD11b Mouse IgG2a Recombinant Antibody (65606-1-MR, Clone: OX42) (red) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue) and FITC anti-Mouse IgG2a Antibody. Cells were not fixed.

  • Flow cytometry (FC) experiment of rat bone marrow cells using Anti-Rat CD11b (OX42) Mouse IgG2a Recombinant Anti (65606-1-MR)

    FC experiment of rat bone marrow cells using 65606-1-MR

    1x10^6 rat bone marrow cells were surface stained with 0.25 ug Anti-Rat CD11b Mouse IgG2a Recombinant Antibody (65606-1-MR, Clone: OX42) (left) or 0.25 ug Anti-Rat CD11b (65229-1-Ig, Clone: OX42) (right) and FITC anti-Mouse IgG2a Antibody. Cells were not fixed.

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Tested Applications

Positive FC detected inrat bone marrow cells

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65606-1-MR targets CD11b in FC applications and shows reactivity with Rat samples.

Tested Reactivity Rat
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Immunogen Rat peritoneal macrophages 相同性解析による交差性が予測される生物種
Full Name integrin alpha M
Calculated molecular weight127 kDa
GenBank accession numberNM_012711
Gene symbol CD11b
Gene ID (NCBI) 25021
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b, also known as Integrin alpha M or CR3A, belongs to the integrin alpha chain family. CD11b forms an α/β heterodimer with CD18 (integrin β2). CD11b/CD18 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PMID: 9558116; 20008295). CD11b/CD18 is a receptor for the complement protein fragment iC3b, and is also a receptor for fibrinogen, factor X and ICAM1 (PMID: 2971974; 15485828).

Protocols

Product Specific Protocols
FC protocol for CD11b antibody 65606-1-MRDownload protocol
Standard Protocols
Click here to view our Standard Protocols