Validation Data Gallery

Filter: AllFC

FC experiment of human PBMCs using 65511-1-MR (same clone as 65511-1-PBS)
1x10^6 human PBMCs were surface stained with 0.4 ug Anti-Human CD11c Mouse Recombinant Antibody (65511-1-MR, Clone:3.9) or Mouse IgG1 Isotype Control, and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were then stained with FITC-conjugated Anti-Human CD14. Cells were not fixed. Cells were treated with FC Receptor Block prior to staining. Monocytes were gated. This data was developed using the same antibody clone with 65511-1-PBS in a different storage buffer formulation.
×
1x10^6 human PBMCs were surface stained with 0.4 ug Anti-Human CD11c Mouse Recombinant Antibody (65511-1-MR, Clone:3.9) or Mouse IgG1 Isotype Control, and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were then stained with FITC-conjugated Anti-Human CD14. Cells were not fixed. Cells were treated with FC Receptor Block prior to staining. Monocytes were gated. This data was developed using the same antibody clone with 65511-1-PBS in a different storage buffer formulation.
FC experiment of human PBMCs using 65511-1-PBS
1x10^6 human PBMCs were surface stained with 0.4 ug Anti-Human CD11c Mouse Recombinant Antibody (65511-1-PBS, Clone:3.9) or Mouse IgG1 Isotype Control, and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were then stained with FITC-conjugated Anti-Human CD14. Cells were not fixed. Cells were treated with FC Receptor Block prior to staining. Monocytes were gated.
×
1x10^6 human PBMCs were surface stained with 0.4 ug Anti-Human CD11c Mouse Recombinant Antibody (65511-1-PBS, Clone:3.9) or Mouse IgG1 Isotype Control, and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were then stained with FITC-conjugated Anti-Human CD14. Cells were not fixed. Cells were treated with FC Receptor Block prior to staining. Monocytes were gated.
FC experiment of human PBMCs using 65511-1-PBS
1x10^6 human PBMCs were surface stained with 0.4 ug Anti-Human CD11c Mouse Recombinant Antibody (65511-1-PBS, Clone:3.9) or Mouse IgG1 Isotype Control, and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were not fixed. Cells were treated with FC Receptor Block prior to staining. Monocytes were gated.
FC experiment of human PBMCs using 65511-1-MR (same clone as 65511-1-PBS)
1x10^6 human PBMCs were surface stained with 0.4 ug Anti-Human CD11c Mouse Recombinant Antibody (65511-1-MR, Clone:3.9) or Mouse IgG1 Isotype Control, and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were not fixed. Cells were treated with FC Receptor Block prior to staining. Monocytes were gated. This data was developed using the same antibody clone with 65511-1-PBS in a different storage buffer formulation.
×
1x10^6 human PBMCs were surface stained with 0.4 ug Anti-Human CD11c Mouse Recombinant Antibody (65511-1-MR, Clone:3.9) or Mouse IgG1 Isotype Control, and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were not fixed. Cells were treated with FC Receptor Block prior to staining. Monocytes were gated. This data was developed using the same antibody clone with 65511-1-PBS in a different storage buffer formulation.
FC experiment of human PBMCs using 65511-1-MR (same clone as 65511-1-PBS)
1x10^6 human PBMCs were surface stained with 0.4 ug Anti-Human CD11c Mouse Recombinant Antibody (65511-1-MR, Clone:3.9) (left and red) or 0.4 ug Anti-Human CD11c Mouse Monoclonal Antibody (Clone:3.9) (center and green), and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were then stained with FITC-conjugated Anti-Human CD14. Cells were not fixed. Cells were treated with FC Receptor Block prior to staining. Monocytes were gated. This data was developed using the same antibody clone with 65511-1-PBS in a different storage buffer formulation.
×
1x10^6 human PBMCs were surface stained with 0.4 ug Anti-Human CD11c Mouse Recombinant Antibody (65511-1-MR, Clone:3.9) (left and red) or 0.4 ug Anti-Human CD11c Mouse Monoclonal Antibody (Clone:3.9) (center and green), and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were then stained with FITC-conjugated Anti-Human CD14. Cells were not fixed. Cells were treated with FC Receptor Block prior to staining. Monocytes were gated. This data was developed using the same antibody clone with 65511-1-PBS in a different storage buffer formulation.
FC experiment of human PBMCs using 65511-1-PBS
1x10^6 human PBMCs were surface stained with 0.4 ug Anti-Human CD11c Mouse Recombinant Antibody (65511-1-PBS, Clone:3.9) (left and red) or 0.4 ug Anti-Human CD11c Mouse Monoclonal Antibody (Clone:3.9) (center and green), and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were then stained with FITC-conjugated Anti-Human CD14. Cells were not fixed. Cells were treated with FC Receptor Block prior to staining. Monocytes were gated.
×
1x10^6 human PBMCs were surface stained with 0.4 ug Anti-Human CD11c Mouse Recombinant Antibody (65511-1-PBS, Clone:3.9) (left and red) or 0.4 ug Anti-Human CD11c Mouse Monoclonal Antibody (Clone:3.9) (center and green), and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were then stained with FITC-conjugated Anti-Human CD14. Cells were not fixed. Cells were treated with FC Receptor Block prior to staining. Monocytes were gated.

Anti-Human CD11c (3.9) Mouse IgG1 Recombinant Antibody, PBS Only

CD11c Recombinant Antibody for FC

Cat No. 65511-1-PBS

Clone No.3.9

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

Synonyms

Leu M5, ITGAX, Integrin alpha-X, Integrin alpha X, CD11 antigen-like family member C

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive FC detected in

human PBMCs

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

65511-1-PBS targets CD11c in FC applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Mouse / IgG1
Class	Recombinant
Type	Antibody
Immunogen	Rheumatoid synovial cells and human monocytes 相同性解析による交差性が予測される生物種
Full Name	integrin, alpha X (complement component 3 receptor 4 subunit)
Calculated molecular weight	1169 aa, 129 kDa
GenBank accession number	BC038237
Gene Symbol	CD11c
Gene ID (NCBI)	3687
ENSEMBL Gene ID	ENSG00000140678
Conjugate	Unconjugated
Form	Liquid
Purification Method	Affinity purification
Storage Buffer	PBS only
Storage Conditions	Store at -80°C. Stable for one year after shipment.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11c, also known as integrin αX, is a type I transmembrane glycoprotein present on a variety of cells, including monocytes/macrophages, granulocytes, a subset of B cells, NK cells and dendritic cells (PMID: 2897326; 1680915; 1694698; 17389580). As a result of its high level of expression on most dendritic cells, CD11c is typically considered to be a marker of conventional dendritic cells (PMID: 27119555). CD11c forms an α/β heterodimer with CD18 (integrin β2). CD11c/CD18 acts a receptor for fibrinogen and is important in monocyte adhesion and chemotaxis (PMID: 1671533).

Protocols

Product Specific Protocols
FC protocol for CD11c antibody 65511-1-PBS	Download protocol

Standard Protocols
Click here to view our Standard Protocols

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Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

FC experiment of human PBMCs using 65511-1-MR (same clone as 65511-1-PBS)

FC experiment of human PBMCs using 65511-1-PBS

FC experiment of human PBMCs using 65511-1-PBS

FC experiment of human PBMCs using 65511-1-MR (same clone as 65511-1-PBS)

FC experiment of human PBMCs using 65511-1-MR (same clone as 65511-1-PBS)

FC experiment of human PBMCs using 65511-1-PBS

Anti-Human CD11c (3.9) Mouse IgG1 Recombinant Antibody, PBS Only

CD11c Recombinant Antibody for FC

-/ -

Tested Applications

Recommended dilution

Product Information

Background Information

Protocols