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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using CD11d/Integrin Alpha D Polyclonal antibody (55162-1-AP)

    WB analysis of Jurkat using 55162-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 55162-1-AP (CD11d antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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CD11d/Integrin Alpha D Polyclonal antibody

CD11d/Integrin Alpha D Polyclonal Antibody for WB, ELISA
Cat No. 55162-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

ADB2, CD11d, Integrin alpha D, integrin, alpha D, ITGAD, Leukointegrin alpha D

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55162-1-AP targets CD11d/Integrin Alpha D in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name integrin, alpha D
Calculated molecular weight 127 kDa
Observed molecular weight 126-150 kDa
GenBank accession numberNM_005353
Gene Symbol CD11d
Gene ID (NCBI) 3681
RRIDAB_10860128
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13349
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

CD11d, also named as ITGAD and ADB2, belongs to the integrin alpha chain family. It is a receptor for ICAM3 and VCAM1. CD11d may play a role in the atherosclerotic process such as clearing lipoproteins from plaques and in phagocytosis of blood-borne pathogens, particulate matter, and senescent erythrocytes from the blood. This antibody is specific to CD11d.

Protocols

Product Specific Protocols
WB protocol for CD11d/Integrin Alpha D antibody 55162-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols