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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD122/IL-2RB Monoclonal antibody, PBS Only (Capture)

CD122/IL-2RB Monoclonal Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5G1E6

Cat no : 60174-2-PBS

Synonyms

IL2RB, IL 2RB, IL 2R subunit beta, IL 2 receptor subunit beta, High affinity IL-2 receptor subunit beta

Validation Data Gallery

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  • Western Blot (WB) analysis of Recombinant protein using CD122/IL-2RB Monoclonal antibody (60174-2-Ig)

    WB analysis of Recombinant protein using 60174-2-Ig (same clone as 60174-2-PBS)

    Recombinant protein were subjected to SDS PAGE followed by western blot with 60174-2-Ig (IL2RB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60174-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Recombinant protein using CD122/IL-2RB Monoclonal antibody (60174-2-Ig)

    WB analysis of Recombinant protein using 60174-2-Ig (same clone as 60174-2-PBS)

    Recombinant protein were subjected to SDS PAGE followed by western blot with 60174-2-Ig (IL2RB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60174-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50451-2

    Cytometric bead array standard curve of MP50451-2

    Cytometric bead array standard curve of MP50451-2, CD122/IL2RB Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60174-2-PBS. Detection antibody: 60174-1-PBS. Standard:Ag3929. Range: 0.098-50 ng/mL.

  • Cytometric bead array standard curve of MP50451-3

    Cytometric bead array standard curve of MP50451-3

    Cytometric bead array standard curve of MP50451-3, CD122/IL2RB Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60174-2-PBS. Detection antibody: 60174-3-PBS. Standard:Ag3929. Range: 0.098-3.125 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60174-2-PBS targets CD122/IL-2RB as part of a matched antibody pair:

MP50451-2: 60174-2-PBS capture and 60174-1-PBS detection (validated in Cytometric bead array)

MP50451-3: 60174-2-PBS capture and 60174-3-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CD122/IL-2RB fusion protein Ag3929 相同性解析による交差性が予測される生物種
Full Name interleukin 2 receptor, beta
Calculated molecular weight 551 aa, 61 kDa
GenBank accession numberBC025691
Gene symbol IL2RB
Gene ID (NCBI) 3560
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.