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  • Featured Product
  • KD/KO Validated

CD126/IL-6R alpha Polyclonal antibody, PBS Only

CD126/IL-6R alpha Polyclonal Antibody for WB, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 23457-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using CD126/IL-6R alpha Polyclonal antibody (23457-1-AP)

    WB analysis of Jurkat using 23457-1-AP (same clone as 23457-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 23457-1-AP (IL-6R alpha antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23457-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse spleen tissue using CD126/IL-6R alpha Polyclonal antibody (23457-1-AP)

    WB analysis of mouse spleen using 23457-1-AP (same clone as 23457-1-PBS)

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 23457-1-AP (IL-6R alpha antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23457-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CD126/IL-6R alpha Polyclonal antibody (23457-1-AP)

    WB analysis using 23457-1-AP (same clone as 23457-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 23457-1-AP (IL-6R alpha antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23457-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse spleen tissue using CD126/IL-6R alpha Polyclonal antibody (23457-1-AP)

    IP experiment of mouse spleen using 23457-1-AP (same clone as 23457-1-PBS)

    IP result of anti-IL-6R alpha (IP:23457-1-AP, 4ug; Detection:23457-1-AP 1:500) with mouse spleen tissue lysate 4000ug. This data was developed using the same antibody clone with 23457-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

23457-1-PBS targets CD126/IL-6R alpha in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD126/IL-6R alpha fusion protein Ag18263 相同性解析による交差性が予測される生物種
Full Name interleukin 6 receptor
Calculated molecular weight 468 aa, 52 kDa
Observed molecular weight 80 kDa
GenBank accession numberBC132684
Gene symbol IL-6R alpha
Gene ID (NCBI) 3570
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Interleukin-6 (IL-6) is a pleiotropic cytokine produced by a variety of cells during infection, trauma, and immunological challenge (PMID: 8274730). IL-6 acts via a receptor complex consisting of two distinct membrane-bound glycoproteins, an 80-kDa IL-6-binding subunit (IL-6R alpha, CD126, gp80) and a 130-kDa signal-transducing element (gp130, CD130). After binding of IL-6 to membrane-bound IL-6R alpha, the complex of IL-6 and IL-6R alpha associates with gp130, thus activating the receptor (PMID: 9716487). Expression of gp130 is found in almost all organs while expression of IL-6R alpha is predominantly confined to hepatocytes and leukocyte subpopulations (monocytes, neutrophils, T cells, and B cells) (PMID: 11149892). Soluble form of IL-6R alpha has been found in human serum and urine (PMID: 2529343; 1545125).