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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human CD13 (WM15), PBS Only

CD13 Monoclonal Antibody for IF/ICC, FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human, non-human primates

Applications

IF/ICC, FC

Conjugate

Unconjugated

CloneNo.

WM15

Cat no : 65186-1-PBS

Synonyms

APN, AP-M, ANPEP, Aminopeptidase N, Aminopeptidase M

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HUVEC cells using Anti-Human CD13 (WM15) (65186-1-Ig)

    IF Staining of HUVEC using 65186-1-Ig (same clone as 65186-1-PBS)

    Immunofluorescent analysis of un-fixed HUVEC cells using CD13 antibody (65186-1-Ig, Clone: WM15 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004). This data was developed using the same antibody clone with 65186-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of THP-1 cells using Anti-Human CD13 (WM15) (65186-1-Ig)

    IF Staining of THP-1 using 65186-1-Ig (same clone as 65186-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CD13 antibody (65186-1-Ig, Clone: WM15 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 65186-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD13 (WM15) (65186-1-Ig)

    FC experiment of human PBMCs using 65186-1-Ig (same clone as 65186-1-PBS)

    1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD13 (65186-1-Ig, Clone:WM15) and FITC-Goat anti-Mouse IgG at dilution 1:1000 or unstained. Cells were not fixed. Monocytes were gated. This data was developed using the same antibody clone with 65186-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD13 (WM15) (65186-1-Ig)

    FC experiment of human PBMCs using 65186-1-Ig (same clone as 65186-1-PBS)

    1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD13 (65186-1-Ig, Clone:WM15) and FITC-Goat anti-Mouse IgG at dilution 1:1000 or unstained. Cells were not fixed. Monocytes were gated. This data was developed using the same antibody clone with 65186-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

65186-1-PBS targets CD13 in IF/ICC, FC applications and shows reactivity with human, non-human primates samples.

Tested Reactivity human, non-human primates
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen Human AML cells 相同性解析による交差性が予測される生物種
Full Name alanyl (membrane) aminopeptidase
Calculated molecular weight 110 kDa
GenBank accession numberBC058928
Gene symbol CD13
Gene ID (NCBI) 290
RRIDAB_2918457
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD13, also named as APN, ANPEP (aminopeptidase N) or PEPN, is belongs to the peptidase M1 family. CD13 is a heavily glycosylated, ~150-240 kDa, type-II membrane, expressed by most cells of myeloid origin including monocytes, macrophages, granulocytes, and their hematopoietic precursors. It is also abundantly expressed in the brush border of epithelial cells from renal proximal tubules and small intestine, in prostatic epithelial cells, in bile duct canaliculi, in mast cells, and, in some cases, in fibroblasts and smooth muscle cells. CD13 is a multifunctional protein and plays varying roles in cell migration, cell proliferation, cell differentiation and so on. CD13 participates in angiogenesis generating and modulating angiogenic signals, and can be a marker of angiogenic vessels. CD13 is also a pan-myeloid marker, present on mature granulocytes and monocytes. (PMID: 8805662, 10098327, 18603472, 18097955, 17897790, 17888402, 21339174)