• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human CD13 (WM15) Mouse IgG2a Recombinant Antibody, PBS Only

CD13 Recombinant Antibody for IF/ICC, FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF/ICC, FC

Conjugate

Unconjugated

CloneNo.

WM15

Cat no : 65633-1-PBS

Synonyms

APN, AP-M, ANPEP, Aminopeptidase N, Aminopeptidase M

Validation Data Gallery

Filter:
  • Immunofluorescence (IF) / fluorescent staining of THP-1 cells using Anti-Human CD13 (WM15) Mouse IgG2a Recombinant Ant (65633-1-MR)

    IF Staining of THP-1 using 65633-1-MR (same clone as 65633-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CD13 antibody (65633-1-MR, Clone: WM15 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 65633-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD13 (WM15) Mouse IgG2a Recombinant Ant (65633-1-MR)

    FC experiment of human PBMCs using 65633-1-MR (same clone as 65633-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD13 (WM15) Mouse IgG2a Recombinant Antibody (65633-1-MR, Clone: WM15) or Mouse IgG2a Isotype Control, and PE-conjugated Goat Anti-Mouse IgG. Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were not fixed. Monocytes were gated. This data was developed using the same antibody clone with 65633-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD13 (WM15) Mouse IgG2a Recombinant Ant (65633-1-MR)

    FC experiment of human PBMCs using 65633-1-MR (same clone as 65633-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD13 (WM15) Mouse IgG2a Recombinant Antibody (65633-1-MR, Clone: WM15) (red) or Mouse IgG2a Isotype Control (blue), and PE-conjugated Goat Anti-Mouse IgG. Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were not fixed. Monocytes were gated. This data was developed using the same antibody clone with 65633-1-PBS in a different storage buffer formulation.

☆大好評トライアルサイズ ¥20,000～！ >>> 一次抗体特別価格キャンペーン開催中！

Tested Applications

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

65633-1-PBS targets CD13 in IF/ICC, FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Immunogen Human AML cells 相同性解析による交差性が予測される生物種
Full Name alanyl (membrane) aminopeptidase
Calculated molecular weight 110 kDa
GenBank accession numberBC058928
Gene symbol CD13
Gene ID (NCBI) 290
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD13, also named as APN, ANPEP (aminopeptidase N) or PEPN, is belongs to the peptidase M1 family. CD13 is a heavily glycosylated, ~150-240 kDa, type-II membrane, expressed by most cells of myeloid origin including monocytes, macrophages, granulocytes, and their hematopoietic precursors. It is also abundantly expressed in the brush border of epithelial cells from renal proximal tubules and small intestine, in prostatic epithelial cells, in bile duct canaliculi, in mast cells, and, in some cases, in fibroblasts and smooth muscle cells. CD13 is a multifunctional protein and plays varying roles in cell migration, cell proliferation, cell differentiation and so on. CD13 participates in angiogenesis generating and modulating angiogenic signals, and can be a marker of angiogenic vessels. CD13 is also a pan-myeloid marker, present on mature granulocytes and monocytes. (PMID: 8805662, 10098327, 18603472, 18097955, 17897790, 17888402, 21339174)