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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD133 Monoclonal antibody, PBS Only

CD133 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2B8A2

Cat no : 66666-1-PBS

Synonyms

MSTP061, MCDR2, CORD12, Antigen AC133, AC133

Validation Data Gallery

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  • Western Blot (WB) analysis of HT-29 cells using CD133 Monoclonal antibody (66666-1-Ig)

    WB analysis of HT-29 using 66666-1-Ig (same clone as 66666-1-PBS)

    Untreated and PNGase F-treated lysates of HT-29 cells and Caco-2 cells were subjected to SDS PAGE followed by western blot with 66666-1-Ig (CD133 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808). This data was developed using the same antibody clone with 66666-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using CD133 Monoclonal antibody (66666-1-Ig)

    IHC staining of human kidney using 66666-1-Ig (same clone as 66666-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66666-1-Ig (CD133 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66666-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using CD133 Monoclonal antibody (66666-1-Ig)

    IHC staining of human kidney using 66666-1-Ig (same clone as 66666-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66666-1-Ig (CD133 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66666-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CD133 Monoclonal antibody (66666-1-Ig)

    IHC staining of human breast cancer using 66666-1-Ig (same clone as 66666-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66666-1-Ig (CD133 antibody) at dilution of 1:2100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66666-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CD133 Monoclonal antibody (66666-1-Ig)

    IHC staining of human breast cancer using 66666-1-Ig (same clone as 66666-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66666-1-Ig (CD133 antibody) at dilution of 1:2100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66666-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CD133 Monoclonal antibody (66666-1-Ig)

    IHC staining of human colon cancer using 66666-1-Ig (same clone as 66666-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66666-1-Ig (CD133 antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66666-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CD133 Monoclonal antibody (66666-1-Ig)

    IHC staining of human colon cancer using 66666-1-Ig (same clone as 66666-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66666-1-Ig (CD133 antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66666-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CD133 Monoclonal antibody (66666-1-Ig)

    IHC staining of human breast cancer using 66666-1-Ig (same clone as 66666-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66666-1-Ig (CD133 antibody) at dilution of 1:2100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66666-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CD133 Monoclonal antibody (66666-1-Ig)

    IHC staining of human breast cancer using 66666-1-Ig (same clone as 66666-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66666-1-Ig (CD133 antibody) at dilution of 1:2100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66666-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse colon tissue using CD133 Monoclonal antibody (66666-1-Ig)

    IF Staining of mouse colon using 66666-1-Ig (same clone as 66666-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse colon tissue using CD133 antibody (66666-1-Ig, Clone: 2B8A2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66666-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66666-1-PBS targets CD133 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD133 fusion protein Ag13327 相同性解析による交差性が予測される生物種
Full Name prominin 1
Calculated molecular weight 97 kDa
Observed molecular weight 115 kDa, 80-90 kDa
GenBank accession numberBC012089
Gene symbol CD133
Gene ID (NCBI) 8842
RRIDAB_2801586
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD133, also known as PROM1 (prominin-1) or AC133, belongs to the prominin family. CD133 is a transmembrane glycoprotein with an NH2-terminal extracellular domain, five transmembrane loops and a cytoplasmic tail. The expression of CD133 has been reported in hematopoietic stem cells, endothelial progenitor cells, neuronal and glial stem cells, suggesting the potential role of CD133 as a cell surface marker of adult stem cells. CD133 has also been reported as a marker of cancer stem cells in various human tumors. CD133 is a highly glycosylated protein with an apparent molecular weight of 115-120 kDa. After the treatment of the lysates with glycosidase, CD133 shifted to a protein with an apparent molecular weight of 80-90 kDa (PMID: 23150174; 20068153).