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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD133 Monoclonal antibody, PBS Only

CD133 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2B8A2

Cat no : 66666-1-PBS

Synonyms

AC133, Antigen AC133, CD133, CORD12, MCDR2, MSTP061, PROM1, PROM1 1, PROM1 1,2,3, PROM1 1,2,3,5,7, PROM1 1,6,7, PROM1 2, PROM1 2,3,4,5, prominin 1, Prominin like protein 1, PROML1, RP41, STGD4

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HT-29 cells using CD133 Monoclonal antibody (66666-1-Ig)

    WB analysis of HT-29 using 66666-1-Ig (same clone as 66666-1-PBS)

    Untreated and PNGase F-treated lysates of HT-29 cells and Caco-2 cells were subjected to SDS PAGE followed by western blot with 66666-1-Ig (CD133 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808). This data was developed using the same antibody clone with 66666-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using CD133 Monoclonal antibody (66666-1-Ig)

    IHC staining of human kidney using 66666-1-Ig (same clone as 66666-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66666-1-Ig (CD133 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66666-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using CD133 Monoclonal antibody (66666-1-Ig)

    IHC staining of human kidney using 66666-1-Ig (same clone as 66666-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66666-1-Ig (CD133 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66666-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CD133 Monoclonal antibody (66666-1-Ig)

    IHC staining of human breast cancer using 66666-1-Ig (same clone as 66666-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66666-1-Ig (CD133 antibody) at dilution of 1:2100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66666-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CD133 Monoclonal antibody (66666-1-Ig)

    IHC staining of human breast cancer using 66666-1-Ig (same clone as 66666-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66666-1-Ig (CD133 antibody) at dilution of 1:2100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66666-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CD133 Monoclonal antibody (66666-1-Ig)

    IHC staining of human colon cancer using 66666-1-Ig (same clone as 66666-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66666-1-Ig (CD133 antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66666-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CD133 Monoclonal antibody (66666-1-Ig)

    IHC staining of human colon cancer using 66666-1-Ig (same clone as 66666-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66666-1-Ig (CD133 antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66666-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CD133 Monoclonal antibody (66666-1-Ig)

    IHC staining of human breast cancer using 66666-1-Ig (same clone as 66666-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66666-1-Ig (CD133 antibody) at dilution of 1:2100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66666-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CD133 Monoclonal antibody (66666-1-Ig)

    IHC staining of human breast cancer using 66666-1-Ig (same clone as 66666-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66666-1-Ig (CD133 antibody) at dilution of 1:2100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66666-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66666-1-PBS targets CD133 in WB, IHC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD133 fusion protein Ag13327 相同性解析による交差性が予測される生物種
Full Name prominin 1
Calculated molecular weight 97 kDa
Observed molecular weight 115 kDa, 80-90 kDa
GenBank accession numberBC012089
Gene symbol CD133
Gene ID (NCBI) 8842
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD133, also known as PROM1 (prominin-1) or AC133, belongs to the prominin family. CD133 is a transmembrane glycoprotein with an NH2-terminal extracellular domain, five transmembrane loops and a cytoplasmic tail. The expression of CD133 has been reported in hematopoietic stem cells, endothelial progenitor cells, neuronal and glial stem cells, suggesting the potential role of CD133 as a cell surface marker of adult stem cells. CD133 has also been reported as a marker of cancer stem cells in various human tumors. CD133 is a highly glycosylated protein with an apparent molecular weight of 115-120 kDa. After the treatment of the lysates with glycosidase, CD133 shifted to a protein with an apparent molecular weight of 80-90 kDa (PMID: 23150174; 20068153).