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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD134/OX40 Polyclonal antibody

CD134/OX40 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 29361-1-AP

Synonyms

ACT35, ACT35 antigen, CD134, CD134/OX40, OX40, OX40L receptor, TNFRSF4, TXGP1L

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD134/OX40 Polyclonal antibody (29361-1-AP)

    WB analysis using 29361-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29361-1-AP (CD134/OX40 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inMJ cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29361-1-AP targets CD134/OX40 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD134/OX40 fusion protein Ag28521 相同性解析による交差性が予測される生物種
Full Name tumor necrosis factor receptor superfamily, member 4
Calculated molecular weight 29 kDa
Observed molecular weight50 kDa
GenBank accession numberNM_003327
Gene symbol CD134
Gene ID (NCBI) 7293
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD134, also known as OX40 and TNFRSF4, is a member of the TNFR-superfamily of receptors (PMID: 2828930; 9766631). It is a type I transmembrane protein predominantly expressed on activated T cells which include CD4 and CD8 T cells, Th2, Th1, and Th17 cells, as well as regulatory T cells (Tregs) (PMID: 20307208). CD134 is activated by its cognate ligand CD134L (OX40L) and functions as a T cell co-stimulatory molecule (PMID: 26215166). CD134-CD134L interactions have been proposed as a potential therapeutic target for treating autoimmune diseases, cancer and infectious disease (PMID: 26215166; 19426222).

Protocols

Product Specific Protocols
WB protocol for CD134/OX40 antibody 29361-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols