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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD134/OX40 Polyclonal antibody

CD134/OX40 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 31805-1-AP

Synonyms

TNFRSF4, TAX transcriptionally-activated glycoprotein 1 receptor, OX40L receptor, OX40, CD134

Validation Data Gallery

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  • Western Blot (WB) analysis of MJ cells using CD134/OX40 Polyclonal antibody (31805-1-AP)

    WB analysis of MJ using 31805-1-AP

    MJ cells were subjected to SDS PAGE followed by western blot with 31805-1-AP (CD134/OX40 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD134/OX40 Polyclonal antibody (31805-1-AP)

    IHC staining of human tonsillitis using 31805-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 31805-1-AP (CD134/OX40 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inMJ cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31805-1-AP targets CD134/OX40 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name tumor necrosis factor receptor superfamily, member 4
Calculated molecular weight 29 kDa
Observed molecular weight50 kDa
GenBank accession numberNM_003327
Gene symbol CD134
Gene ID (NCBI) 7293
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD134 (OX40) is a member of the TNFR-superfamily of receptors. Predominantly expressed on activated T cells, CD134 is activated by its cognate ligand CD134L (OX40L) and functions as a T cell co-stimulatory molecule. CD134-CD134L interactions have been proposed as a potential therapeutic target for treating autoimmunity (PMID: 26215166). CD134 can interact with TRAF2, TRAF3, and TRAF5.

Protocols

Product Specific Protocols
WB protocol for CD134/OX40 antibody 31805-1-APDownload protocol
IHC protocol for CD134/OX40 antibody 31805-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols