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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human CD137 (4B4-1)

CD137 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human, non-human primates

Applications

FC

Conjugate

Unconjugated

CloneNo.

4B4-1

Cat no : 65159-1-Ig

Synonyms

TNFRSF9, T cell antigen 4 1BB homolog, ILA, 4B4-1, 4-1BB ligand receptor

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human peripheral blood mononuclear cells using Anti-Human CD137 (4B4-1) (65159-1-Ig)

    FC experiment of human peripheral blood mononuclear cells using 65159-1-Ig

    1X10^6 PHA stimulated (5 μg/mL, 3 days) (right) or unstimulated (left) human peripheral blood mononuclear cells (PBMCs) were surface stained with 0.13 ug Anti-Human CD137 (65159-1-Ig, Clone: 4B4-1) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were not fixed.

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Tested Applications

Positive FC detected inhuman peripheral blood mononuclear cells

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65159-1-Ig targets CD137 in FC applications and shows reactivity with human, non-human primates samples.

Tested Reactivity human, non-human primates
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen Recombinant human CD137 ectodomain 相同性解析による交差性が予測される生物種
Full Name tumor necrosis factor receptor superfamily, member 9
Calculated molecular weight 28 kDa
GenBank accession numberBC006196
Gene symbol TNFRSF9/CD137
Gene ID (NCBI) 3604
RRIDAB_2918441
Conjugate Unconjugated
Form Liquid
Purification MethodPurified by protein-A affinity chromatography
Storage Buffer PBS with 0.1% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

CD137, also known as TNFRSF9 or 4-1BB, is an inducible T cell surface receptor that belongs to the tumor necrosis factor receptor superfamily. CD137 is a transmembrane protein expressed on the surface of activated T-cells. In addition, activation-dependent expression of CD137 has also been found in B lymphocytes, monocytes, and diverse nonlymphoid cell types. CD137 provides a co-stimulatory signal that enhances the survival, and differentiation of cells, and has a crucial role in the development of CD8 cytotoxic T cells and anti-tumor immunity. (PMID: 9826581; 23696891)

Protocols

Product Specific Protocols
FC protocol for CD137 antibody 65159-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols