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Validation Data Gallery

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  • Flow cytometry (FC) experiment of human peripheral blood mononuclear cells using PE Anti-Human CD137 (4B4-1) (PE-65159)

    FC experiment of human peripheral blood mononuclear cells using PE-65159

    1X10^6 PHA-stimulated (5 μg/mL, 3 days) (red) or unstimulated (blue) human peripheral blood mononuclear cells (PBMCs) were surface stained with 10.00 ul PE Anti-Human CD137 (PE-65159, Clone: 4B4-1). Cells were not fixed.

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PE Anti-Human CD137 (4B4-1)

CD137 Monoclonal Antibody for FC
Cat No. PE-65159
Clone No.4B4-1

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human, Non-human primates

Applications

FC

Synonyms

4 1BB, 4 1BB ligand receptor, CD137, CDw137, ILA, T cell antigen 4 1BB homolog, T cell antigen ILA, TNFRSF9

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  {{ptg:cur_Conjugation}}
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive FC detected inhuman peripheral blood mononuclear cells

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 10 µl per 10^6 cells in a 100 µl suspension or 10 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

PE-65159 targets CD137 in FC applications and shows reactivity with Human, Non-human primates samples.

Tested Reactivity Human, Non-human primates
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen Recombinant human CD137 ectodomain 相同性解析による交差性が予測される生物種
Full Name tumor necrosis factor receptor superfamily, member 9
Calculated molecular weight 28 kDa
GenBank accession numberBC006196
Gene Symbol TNFRSF9/CD137
Gene ID (NCBI) 3604
RRIDAB_2883937
Conjugate PE Fluorescent Dye
Excitation/Emission maxima wavelengths496 nm, 565 nm / 578 nm
Form Liquid
Purification MethodThe purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
UNIPROT IDQ07011
Storage Buffer PBS with 0.09% sodium azide
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Protocols

Product Specific Protocols
FC protocol for PE CD137 antibody PE-65159Download protocol
Standard Protocols
Click here to view our Standard Protocols