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Species Reactivity

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  • Featured Product
  • KD/KO Validated

CD138/Syndecan-1 Polyclonal antibody, PBS Only

CD138/Syndecan-1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 10593-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD138/Syndecan-1 Polyclonal antibody (10593-1-AP)

    WB analysis using 10593-1-AP (same clone as 10593-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10593-1-AP (CD138/Syndecan-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10593-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse spleen tissue using CD138/Syndecan-1 Polyclonal antibody (10593-1-AP)

    WB analysis of mouse spleen using 10593-1-AP (same clone as 10593-1-PBS)

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 10593-1-AP (CD138/Syndecan-1 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10593-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD138/Syndecan-1 Polyclonal antibody (10593-1-AP)

    IHC staining of human tonsillitis using 10593-1-AP (same clone as 10593-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10593-1-AP (CD138/Syndecan-1 antibody) at dilution of 1:1600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10593-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD138/Syndecan-1 Polyclonal antibody (10593-1-AP)

    IHC staining of human tonsillitis using 10593-1-AP (same clone as 10593-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10593-1-AP (CD138/Syndecan-1 antibody) at dilution of 1:1600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10593-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using CD138/Syndecan-1 Polyclonal antibody (10593-1-AP)

    IHC staining of human ovary tumor using 10593-1-AP (same clone as 10593-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10593-1-AP (CD138/Syndecan-1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 10593-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10593-1-PBS targets CD138/Syndecan-1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD138/Syndecan-1 fusion protein Ag0823 相同性解析による交差性が予測される生物種
Full Name syndecan 1
Calculated molecular weight 32.5 kDa
Observed molecular weight60-100 kDa, 130 kDa
GenBank accession numberBC008765
Gene symbol CD138
Gene ID (NCBI) 6382
RRIDAB_2182736
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD138, also named as Syndecan-1 (SDC1), is an integral membrane protein. It participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. It is a heparan sulfate proteoglycan expressed on the surface of, and actively shed by, myeloma cells. Altered syndecan-1 expression has been detected in several different tumor types. CD138 was regarded as a useful marker for labeling normal and neoplastic plasma cells and plasmacytoid lymphomas. CD138 has a calculated molecular weight of 32.5 kDa, the apparent molecular weight is higher due to extensive glycosylation.