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Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD14 Monoclonal antibody, PBS Only (Capture)

CD14 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2C1D9

Cat no : 60253-1-PBS

Synonyms

Myeloid cell-specific leucine-rich glycoprotein, Monocyte differentiation antigen CD14, urinary form, Monocyte differentiation antigen CD14, membrane-bound form, CD14 molecule, 2C1D9

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD14 Monoclonal antibody (60253-1-Ig)

    WB analysis using 60253-1-Ig (same clone as 60253-1-PBS)

    Untreated and PNGase F-treated lysates of human milk were subjected to SDS PAGE followed by western blot with 60253-1-Ig (CD14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60253-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of THP-1 cells using CD14 Monoclonal antibody (60253-1-Ig)

    WB analysis of THP-1 using 60253-1-Ig (same clone as 60253-1-PBS)

    THP-1 cells were subjected to SDS PAGE followed by western blot with 60253-1-Ig (CD14 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60253-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using CD14 Monoclonal antibody (60253-1-Ig)

    WB analysis of human placenta using 60253-1-Ig (same clone as 60253-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 60253-1-Ig (CD14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60253-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of RAW 264.7 cells using CD14 Monoclonal antibody (60253-1-Ig)

    WB analysis of RAW 264.7 using 60253-1-Ig (same clone as 60253-1-PBS)

    RAW264.7 cells were subjected to SDS PAGE followed by western blot with 60253-1-Ig (CD14 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60253-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD14 Monoclonal antibody (60253-1-Ig)

    IHC staining of human tonsillitis using 60253-1-Ig (same clone as 60253-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60253-1-Ig (CD14 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60253-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD14 Monoclonal antibody (60253-1-Ig)

    IHC staining of human tonsillitis using 60253-1-Ig (same clone as 60253-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60253-1-Ig (CD14 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60253-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CD14 Monoclonal antibody (60253-1-Ig)

    IF Staining of human tonsillitis using 60253-1-Ig (same clone as 60253-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD14 antibody (60253-1-Ig, Clone: 2C1D9 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60253-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of RAW 264.7 cells using CD14 Monoclonal antibody (60253-1-Ig)

    IF Staining of RAW 264.7 using 60253-1-Ig (same clone as 60253-1-PBS)

    Immunofluorescent analysis of RAW 264.7 cells using 60253-1-Ig (CD14 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L). This data was developed using the same antibody clone with 60253-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50282-2

    Cytometric bead array standard curve of MP50282-2

    Cytometric bead array standard curve of MP50282-2, CD14 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60253-1-PBS. Detection antibody: 60253-2-PBS. Standard:Ag10693. Range: 1.563-200 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60253-1-PBS targets CD14 as part of a matched antibody pair:

MP50282-2: 60253-1-PBS capture and 60253-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD14 fusion protein Ag10693 相同性解析による交差性が予測される生物種
Full Name CD14 molecule
Calculated molecular weight 375 aa, 40 kDa
Observed molecular weight 45-55 kDa
GenBank accession numberBC010507
Gene symbol CD14
Gene ID (NCBI) 929
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD14 is a 50-55 kDa glycosylphosphatidylinositol-anchored glycoprotein preferentially expressed on monocytes and macrophages, and at lower levels on granulocytes (PMID: 3385210; 2462937; 7685797). CD14 can also exist as a soluble protein. CD14 acts as a co-receptor for bacterial liposaccharides (LPS) (PMID: 1698311). It plays a major role in the inflammatory response of monocytes to LPS.