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Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD14 (26IC) (65246-1-Ig)

    FC experiment of human PBMCs using 65246-1-Ig

    1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD14 (65246-1-Ig, Clone:26IC) and APC-conjugated Goat Anti-Mouse IgG at dilution 1:1000 (red) or unstained. Cells were not fixed. Monocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD14 (26IC) (65246-1-Ig)

    FC experiment of human PBMCs using 65246-1-Ig

    1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD14 (65246-1-Ig, Clone:26IC) and APC-conjugated Goat Anti-Mouse IgG at dilution 1:1000 or unstained. Cells were not fixed. Monocytes were gated.

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Anti-Human CD14 (26IC)

CD14 Monoclonal Antibody for FC, ELISA
Cat No. 65246-1-Ig
Clone No.26IC

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

FC, ELISA

Synonyms

CD14, CD14 molecule

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65246-1-Ig targets CD14 in FC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen N/A 相同性解析による交差性が予測される生物種
Full Name CD14 molecule
Calculated molecular weight 375 aa, 40 kDa
GenBank accession numberBC010507
Gene Symbol CD14
Gene ID (NCBI) 929
ENSEMBL Gene IDENSG00000170458
RRIDAB_2935135
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
UNIPROT IDP08571
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

CD14 is a 50-55 kDa glycosylphosphatidylinositol-anchored glycoprotein preferentially expressed on monocytes and macrophages, and at lower levels on granulocytes (PMID: 3385210; 2462937; 7685797). CD14 can also exist as a soluble protein. CD14 acts as a co-receptor for bacterial liposaccharides (LPS) (PMID: 1698311). It plays a major role in the inflammatory response of monocytes to LPS.

Protocols

Product Specific Protocols
FC protocol for CD14 antibody 65246-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols