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Species Reactivity
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Species Reactivity
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD14 Recombinant antibody

CD14 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

230332D4

Cat no : 83055-6-RR

Synonyms

Myeloid cell-specific leucine-rich glycoprotein, Monocyte differentiation antigen CD14, urinary form, Monocyte differentiation antigen CD14, membrane-bound form, CD14 molecule, 230332D4

Validation Data Gallery

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  • Western Blot (WB) analysis of HL-60 cells using CD14 Recombinant antibody (83055-6-RR)

    WB analysis of HL-60 using 83055-6-RR

    HL-60 cells were subjected to SDS PAGE followed by western blot with 83055-6-RR (CD14 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83055-6-RR

    Affinity and Kinetic Characterization of 83055-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83055-6-RR against Human CD14 were performed. The affinity constant is 1.43 nM.

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Tested Applications

Positive WB detected inHL-60 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83055-6-RR is CD14 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name CD14 molecule
Calculated molecular weight 375 aa, 40 kDa
Observed molecular weight50 kDa
GenBank accession numberBC010507
Gene symbol CD14
Gene ID (NCBI) 929
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD14 is a 50-55 kDa glycosylphosphatidylinositol-anchored glycoprotein preferentially expressed on monocytes and macrophages, and at lower levels on granulocytes (PMID: 3385210; 2462937; 7685797). CD14 can also exist as a soluble protein. CD14 acts as a co-receptor for bacterial liposaccharides (LPS) (PMID: 1698311). It plays a major role in the inflammatory response of monocytes to LPS.

Protocols

Product Specific Protocols
WB protocol for CD14 antibody 83055-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols