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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Mouse CD14 Rabbit Recombinant Antibody, PBS Only

CD14 Recombinant Antibody for IF/ICC, FC

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

IF/ICC, FC

Conjugate

Unconjugated

CloneNo.

240225C2

Cat no : 98020-1-PBS

Synonyms

CD14 antigen, 240225C2

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of RAW 264.7 cells using Anti-Mouse CD14 Rabbit Recombinant Antibody (98020-1-RR)

    IF Staining of RAW 264.7 using 98020-1-RR (same clone as 98020-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using CD14 antibody (98020-1-RR, Clone: 240225C2 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 98020-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of mouse peritoneal macrophages using Anti-Mouse CD14 Rabbit Recombinant Antibody (98020-1-RR)

    FC experiment of mouse peritoneal macrophages using 98020-1-RR (same clone as 98020-1-PBS)

    1x10^6 mouse peritoneal macrophages were surface stained with 0.25 ug Anti-Mouse CD14 Rabbit Recombinant Antibody (98020-1-RR, Clone: 240225C2) and PE-conjugated Goat Anti-Rabbit IgG (red), or 0.25 ug Isotype Control and PE-conjugated Goat Anti-Rabbit IgG (blue). Cells were not fixed. This data was developed using the same antibody clone with 98020-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 98020-1-RR

    Affinity and Kinetic Characterization of 98020-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98020-1-RR against Mouse CD14 were performed. The affinity constant is 0.383 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 98020-1-PBS is CD14 Fusion Protein expressed in E. coli.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name CD14 antigen
Calculated molecular weight39 kDa
GenBank accession numberNM_009841.4
Gene symbol Cd14
Gene ID (NCBI) 12475
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD14 is a 50-55 kDa glycosylphosphatidylinositol-anchored glycoprotein preferentially expressed on monocytes and macrophages, and at lower levels on granulocytes (PMID: 3385210; 2462937; 7685797). CD14 can also exist as a soluble protein. CD14 acts as a co-receptor for bacterial liposaccharides (LPS) (PMID: 1698311). It plays a major role in the inflammatory response of monocytes to LPS.