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Validation Data Gallery

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  • Western Blot (WB) analysis of A375 cells using CD146/MCAM Monoclonal antibody (66153-1-Ig)

    WB analysis of A375 using 66153-1-Ig (same clone as 66153-1-PBS)

    A375 cells were subjected to SDS PAGE followed by western blot with 66153-1-Ig (CD146/MCAM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66153-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using CD146/MCAM Monoclonal antibody (66153-1-Ig)

    WB analysis of HepG2 using 66153-1-Ig (same clone as 66153-1-PBS)

    Untreated and PNGase F-treated lysates of HepG2 cells and L02 cells were subjected to SDS PAGE followed by western blot with 66153-1-Ig (CD146/MCAM antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808). This data was developed using the same antibody clone with 66153-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using CD146/MCAM Monoclonal antibody (66153-1-Ig)

    WB analysis of HeLa using 66153-1-Ig (same clone as 66153-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66153-1-Ig (CD146/MCAM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66153-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CD146/MCAM Monoclonal antibody (66153-1-Ig)

    WB analysis using 66153-1-Ig (same clone as 66153-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66153-1-Ig (CD146/MCAM antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66153-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CD146/MCAM Monoclonal antibody (66153-1-Ig)

    IHC staining of human liver cancer using 66153-1-Ig (same clone as 66153-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66153-1-Ig (CD146/MCAM antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66153-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CD146/MCAM Monoclonal antibody (66153-1-Ig)

    IHC staining of human liver cancer using 66153-1-Ig (same clone as 66153-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66153-1-Ig (CD146/MCAM antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66153-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human rectal cancer tissue using CD146/MCAM Monoclonal antibody (66153-1-Ig)

    IHC staining of human rectal cancer using 66153-1-Ig (same clone as 66153-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 66153-1-Ig (CD146/MCAM antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66153-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using CD146/MCAM Monoclonal antibody (66153-1-Ig)

    IHC staining of human placenta using 66153-1-Ig (same clone as 66153-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66153-1-Ig (CD146/MCAM antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66153-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using CD146/MCAM Monoclonal antibody (66153-1-Ig)

    IHC staining of human placenta using 66153-1-Ig (same clone as 66153-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66153-1-Ig (CD146/MCAM antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66153-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using CD146/MCAM Monoclonal antibody (66153-1-Ig)

    IF Staining of human liver cancer using 66153-1-Ig (same clone as 66153-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CD146/MCAM antibody (66153-1-Ig, Clone: 4D8A9 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66153-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using CD146/MCAM Monoclonal antibody (66153-1-Ig)

    IF Staining of human liver cancer using 66153-1-Ig (same clone as 66153-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CD146/MCAM antibody (66153-1-Ig, Clone: 4D8A9 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66153-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human placenta tissue using CD146/MCAM Monoclonal antibody (66153-1-Ig)

    IF Staining of human placenta using 66153-1-Ig (same clone as 66153-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using CD146/MCAM antibody (66153-1-Ig, Clone: 4D8A9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66153-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HUVEC cells using CD146/MCAM Monoclonal antibody (66153-1-Ig)

    IF Staining of HUVEC using 66153-1-Ig (same clone as 66153-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using CD146/MCAM antibody (66153-1-Ig, Clone: 4D8A9 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66153-1-PBS in a different storage buffer formulation.

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CD146/MCAM Monoclonal antibody, PBS Only

CD146/MCAM Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA
Cat No. 66153-1-PBS
Clone No.4D8A9

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Synonyms

CD146, 4D8A9, MUC18, Melanoma-associated antigen MUC18, Melanoma-associated antigen A32

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66153-1-PBS targets CD146/MCAM in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD146/MCAM fusion protein Ag11855 相同性解析による交差性が予測される生物種
Full Name melanoma cell adhesion molecule
Calculated molecular weight 646 aa, 72 kDa
Observed molecular weight 120 kDa
GenBank accession numberBC056418
Gene Symbol CD146
Gene ID (NCBI) 4162
RRIDAB_2881549
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP43121
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

CD146, also known as melanoma cell adhesion molecule (MCAM) or MUC18, originally identified as a biomarker of melanoma progression, is a transmembrane glycoprotein of 113-130 kDa, belonging to the immunoglobulin (Ig) superfamily (PMID: 8378324; 25993332). Structurally, it consists of five Ig domains, a transmembrane domain, and a cytoplasmic region. In normal adult tissue, CD146 is primarily expressed by vascular endothelium and smooth muscle. CD146 is a key cell adhesion protein in vascular endothelial cell activity and angiogenesis, and has been used as marker of circulating endothelium cells (CECs) (PMID: 19356677). In addition to the membrane-anchored form of CD146, a soluble form of CD146 (sCD146, 105 kDa) has also been found in human plasma and in the supernatant of cultured human endothelial cells (PMID: 9462829; 19229070; 16374253; 14597988). This antibody detects a band at approximately 120 kDa that corresponds to the molecular weight of glycosylated CD146. Treatment of lysates of HepG2 cells and L02 cells with PNGase F, which removes oligosaccharides from N-linked glycoproteins, led to a down-shift of the detected band.