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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CD147 Recombinant antibody (82909-1-RR)

    IF Staining of HeLa using 82909-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CD147 antibody (82909-1-RR, Clone: 230046C1 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CD147 Recombinant antibody (82909-1-RR)

    IF Staining of MCF-7 using 82909-1-RR

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CD147 antibody (82909-1-RR, Clone: 230046C1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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CD147 Recombinant antibody

CD147 Recombinant Antibody for IF/ICC, ELISA
Cat No. 82909-1-RR
Clone No.230046C1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Synonyms

EMMPRIN, Collagenase stimulatory factor, BSG, Basigin, 5F7

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHeLa cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82909-1-RR targets CD147 in IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name basigin (Ok blood group)
Calculated molecular weight 385 aa, 42 kDa
GenBank accession numberBC009040
Gene Symbol CD147
Gene ID (NCBI) 682
RRIDAB_3670638
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP35613
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD147, also known as Basigin or extracellular matrix metalloproteinase inducer (EMMPRIN), is a transmembrane glycoprotein that belongs to the immunoglobulin superfamily (PMID: 7812975). The molecule is composed of an intracellular portion, an extracellular portion and a single transmembrane region. CD147 is expressed on a variety of cell types (e.g., hematopoietic, epithelial, and endothelial cells) and at varying levels (PMID: 32968061). Increased expression of CD147 occurs in many tumors. CD147 is a pleiotropic molecule that plays an important role in fetal, neuronal, lymphocyte and extracellular matrix development (PMID: 17945211). CD147 has been identified as a receptor essential for erythrocyte invasion by Plasmodium falciparum (PMID: 22080952). It has been reported that spike protein of SARS-CoV-2 binds to CD147 on host cells, thereby mediating the viral invasion (Wang, Ke, et al, BioRxiv, 2020).

Protocols

Product Specific Protocols
IF protocol for CD147 antibody 82909-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols