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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD151 Polyclonal antibody

CD151 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27816-1-AP

Synonyms

CD151, CD151 antigen, GP27, Membrane glycoprotein SFA 1, MER2, PETA 3, RAPH, SFA1, Tetraspanin 24, Tspan 24, TSPAN24

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD151 Polyclonal antibody (27816-1-AP)

    WB analysis using 27816-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27816-1-AP (CD151 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using CD151 Polyclonal antibody (27816-1-AP)

    IHC staining of human ovary tumor using 27816-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 27816-1-AP (CD151 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using CD151 Polyclonal antibody (27816-1-AP)

    IHC staining of human ovary tumor using 27816-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 27816-1-AP (CD151 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using CD151 Polyclonal antibody (27816-1-AP)

    IHC staining of human stomach cancer using 27816-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27816-1-AP (CD151 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using CD151 Polyclonal antibody (27816-1-AP)

    IHC staining of human stomach cancer using 27816-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27816-1-AP (CD151 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse heart tissue, rat heart tissue
Positive IHC detected inhuman ovary tumor tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27816-1-AP targets CD151 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD151 fusion protein Ag25968 相同性解析による交差性が予測される生物種
Full Name CD151 molecule (Raph blood group)
Calculated molecular weight 28 kDa
Observed molecular weight30 kDa
GenBank accession numberBC001374
Gene symbol CD151
Gene ID (NCBI) 977
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD151 (also known as TSPAN24) is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Many of these members are involved in the regulation of cell development, activation, growth and motility. CD151 is broadly expressed by a variety of cell types. It is involved in cellular processes including cell adhesion and may regulate integrin trafficking and/or function. CD151 enhances cell motility, invasion and metastasis of cancer cells.

Protocols

Product Specific Protocols
WB protocol for CD151 antibody 27816-1-APDownload protocol
IHC protocol for CD151 antibody 27816-1-APDownload protocol
FC protocol for CD151 antibody 27816-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Sci Rep

Combined knockdown of CD151 and MMP9 may inhibit the malignant biological behaviours of triple-negative breast cancer through the GSK-3β/β-catenin-related pathway

Authors - Fan Li
View Article