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  • Featured Product
  • KD/KO Validated

CD151 Monoclonal antibody, PBS Only (Capture)

CD151 Monoclonal Antibody for WB, IHC, IP, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IP, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2A8G8

Cat no : 66567-1-PBS

Synonyms

MER2, Membrane glycoprotein SFA-1, Membrane glycoprotein SFA 1, GP27, CD151 antigen

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD151 Monoclonal antibody (66567-1-Ig)

    WB analysis using 66567-1-Ig (same clone as 66567-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66567-1-Ig (CD151 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66567-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of human placenta tissue using CD151 Monoclonal antibody (66567-1-Ig)

    IP experiment of human placenta using 66567-1-Ig (same clone as 66567-1-PBS)

    IP result of anti-CD151 (IP:66567-1-Ig, 4ug; Detection:66567-1-Ig 1:10000) with human placenta tissue lysate 800 ug. This data was developed using the same antibody clone with 66567-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD151 Monoclonal antibody (66567-1-Ig)

    IHC staining of human tonsillitis using 66567-1-Ig (same clone as 66567-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66567-1-Ig (CD151 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66567-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD151 Monoclonal antibody (66567-1-Ig)

    IHC staining of human tonsillitis using 66567-1-Ig (same clone as 66567-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66567-1-Ig (CD151 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66567-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50304-1

    Cytometric bead array standard curve of MP50304-1

    Cytometric bead array standard curve of MP50304-1, CD151 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66567-1-PBS. Detection antibody: 66567-2-PBS. Standard:Ag25985. Range: 0.781-50 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66567-1-PBS targets CD151 as part of a matched antibody pair:

MP50304-1: 66567-1-PBS capture and 66567-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CD151 fusion protein Ag25985 相同性解析による交差性が予測される生物種
Full Name CD151 molecule (Raph blood group)
Calculated molecular weight 28 kDa
Observed molecular weight 28 kDa
GenBank accession numberBC001374
Gene symbol CD151
Gene ID (NCBI) 977
RRIDAB_2881928
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD151 (also known as TSPAN24) is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Many of these members are involved in the regulation of cell development, activation, growth and motility. CD151 is broadly expressed by a variety of cell types. It is involved in cellular processes including cell adhesion and may regulate integrin trafficking and/or function. CD151 enhances cell motility, invasion and metastasis of cancer cells.