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Species Reactivity
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD155/PVR Polyclonal antibody

CD155/PVR Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 31447-1-AP

Synonyms

Pvr, Tage4, poliovirus receptor, CD155

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD155/PVR Polyclonal antibody (31447-1-AP)

    WB analysis using 31447-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31447-1-AP (CD155/PVR antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of RAW 264.7 cells using CD155/PVR Polyclonal antibody (31447-1-AP)

    IF Staining of RAW 264.7 using 31447-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed RAW 264.7 cells using CD155/PVR antibody (31447-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Tested Applications

Positive WB detected inRAW 264.7 cells, mouse liver tissue, mouse small intestine tissue
Positive IF/ICC detected inRAW 264.7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31447-1-AP targets CD155/PVR in WB, IF/ICC, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name poliovirus receptor
Calculated molecular weight45 kDa
Observed molecular weight75-85 kDa
GenBank accession numberNM_027514.2
Gene symbol CD155/PVR
Gene ID (NCBI) 52118
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD155, also known as PVR, is a type I transmembrane glycoprotein in the immunoglobulin superfamily. It contains three extracellular immunoglobulin-like domains, D1-D3, of which D1 is recognized by the virus. CD155 is thought to play a role in adhesion by interaction with the ECM component vitronectin as well as a role in NK killing of tumor cells. CD155 binds to two receptors of NK cells, CD96 and CD226, and accumulates at cell-cell contact sites, leading to the formation of mature immune synapses between NK cells and target cells.

Protocols

Product Specific Protocols
WB protocol for CD155/PVR antibody 31447-1-APDownload protocol
IF protocol for CD155/PVR antibody 31447-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols