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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human CD157 Rabbit Recombinant Antibody, PBS Only

CD157 Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

241424H5

Cat no : 98146-1-PBS

Synonyms

BST 1, Bone marrow stromal antigen 1, ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 2, ADP-ribosyl cyclase 2, ADP ribosyl cyclase 2

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human peripheral blood leukocytes using Anti-Human CD157 Rabbit Recombinant Antibody (98146-1-RR)

    FC experiment of human peripheral blood leukocytes using 98146-1-RR (same clone as 98146-1-PBS)

    1x10^6 human peripheral blood leukocytes were surface stained with 0.15 ug Anti-Human CD157 Rabbit Recombinant Antibody (98146-1-RR, Clone: 241424H5), or Isotype Control and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were not fixed. This data was developed using the same antibody clone with 98146-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of human peripheral blood leukocytes using Anti-Human CD157 Rabbit Recombinant Antibody (98146-1-RR)

    FC experiment of human peripheral blood leukocytes using 98146-1-RR (same clone as 98146-1-PBS)

    1x10^6 human peripheral blood leukocytes were surface stained with 0.15 ug Anti-Human CD157 Rabbit Recombinant Antibody (98146-1-RR, Clone: 241424H5) (red) or Isotype Control (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were not fixed. This data was developed using the same antibody clone with 98146-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 98146-1-RR

    Affinity and Kinetic Characterization of 98146-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98146-1-RR against Human CD157 were performed. The affinity constant is below 1 pM.

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Tested Applications

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

98146-1-PBS targets CD157 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant Protein 相同性解析による交差性が予測される生物種
Full Name bone marrow stromal cell antigen 1
Calculated molecular weight 318 aa, 36 kDa
GenBank accession numberBC012095
Gene symbol CD157
Gene ID (NCBI) 683
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD157, also known as bone marrow stromal cell antigen 1 (BST-1), or ADP-ribosyl cyclase 2, is a glycosyl phosphotidylinositol-anchored protein that stimulates pre-B-cell growth and has adenosine diphosphate (ADP)-ribosyl cyclase and cyclic ADP-ribose (cADPR) hydrolase activity (PMID: 9473467, 11866528). The two enzymatic activities are responsible for the synthesis and hydrolysis of cADPR, a novel second messenger of calcium release from intracellular calcium stores.