CD16 Polyclonal antibody
CD16 Polyclonal Antibody for IHC, IF-P, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (2)
Applications
IHC, IF-P, ELISA
Conjugate
Unconjugated
Cat no : 16559-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive IHC detected in | human liver tissue, human placenta tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | human liver cancer tissue |
Recommended dilution
Application | Dilution |
---|---|
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 6 publications below |
IHC | See 7 publications below |
IF | See 19 publications below |
FC | See 1 publications below |
Product Information
16559-1-AP targets CD16 in IHC, IF-P, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | CD16 fusion protein Ag9787 相同性解析による交差性が予測される生物種 |
Full Name | Fc fragment of IgG, low affinity IIIa, receptor (CD16a) |
Calculated molecular weight | 254 aa, 29 kDa |
Observed molecular weight | 50-55 kDa |
GenBank accession number | BC017865 |
Gene symbol | CD16a |
Gene ID (NCBI) | 2214 |
RRID | AB_2878279 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CD16 is a 50-70-kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD16 has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and the FCGR3B.
Protocols
Product Specific Protocols | |
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IHC protocol for CD16 antibody 16559-1-AP | Download protocol |
IF protocol for CD16 antibody 16559-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Immunother Cancer Discovery of a glucocorticoid receptor (GR) activity signature correlates with immune cell infiltration in adrenocortical carcinoma | ||
J Allergy Clin Immunol Thymic stromal lymphopoietin epigenetically upregulates Fc receptor γ subunit-related receptors on antigen-presenting cells and induces TH2/TH17 polarization through dectin-2. | ||
Theranostics Effects of prenatal photobiomodulation treatment on neonatal hypoxic ischemia in rat offspring. | ||
Theranostics Non-invasive photobiomodulation treatment in an Alzheimer Disease-like transgenic rat model. | ||
J Transl Med Systematic analysis of various RNA transcripts and construction of biological regulatory networks at the post-transcriptional level for chronic obstructive pulmonary disease | ||
Front Immunol Efficacy of MUC1-targeted CAR-NK cells against human tongue squamous cell carcinoma |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Emma (Verified Customer) (11-29-2021) | Works well by IF on FFPE tissue. Antigen retrieval Tris-EDTA pH9.0.
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