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Validation Data Gallery

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  • Flow cytometry (FC) experiment of human peripheral blood leukocyte using Anti-Human CD16 (3G8) Mouse IgG2a Recombinant Anti (65612-1-MR)

    FC experiment of human peripheral blood leukocyte using 65612-1-MR (same clone as 65612-1-PBS)

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD16 Mouse IgG2a Recombinant Antibody (65612-1-MR, Clone: 3G8) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4), and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 65612-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of human peripheral blood leukocyte using Anti-Human CD16 (3G8) Mouse IgG2a Recombinant Anti (65612-1-MR)

    FC experiment of human peripheral blood leukocyte using 65612-1-MR (same clone as 65612-1-PBS)

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD16 Mouse IgG2a Recombinant Antibody (65612-1-MR, Clone:3G8) (red) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 65612-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of human peripheral blood leukocyte using Anti-Human CD16 (3G8) Mouse IgG2a Recombinant Anti (65612-1-MR)

    FC experiment of human peripheral blood leukocyte using 65612-1-MR (same clone as 65612-1-PBS)

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD16 Mouse IgG2a Recombinant Antibody (65612-1-MR, Clone: 3G8) (left) or 0.25 ug Anti-Human CD16 (65090-1-Ig, Clone: 3G8) (right), and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) (right). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 65612-1-PBS in a different storage buffer formulation.

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Anti-Human CD16 (3G8) Mouse IgG2a Recombinant Antibody, PBS Only

CD16 Recombinant Antibody for FC
Cat No. 65612-1-PBS
Clone No.3G8

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Synonyms

Fc gamma RIII alpha, Fc gamma RIII, CD16-II, CD16a antigen, CD16A

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

65612-1-PBS targets CD16 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Immunogen N/A 相同性解析による交差性が予測される生物種
Full Name Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
Calculated molecular weight 254 aa, 29 kDa
GenBank accession numberBC017865
Gene Symbol CD16a
Gene ID (NCBI) 2214
ENSEMBL Gene IDENSG00000203747
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

CD16 is a 50-70-kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD16 has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and the FCGR3B. Clone 3G8 recognizes both the CD16a and CD16b (PMID: 7592758).