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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human liver tissue using CD16 Monoclonal antibody (66779-1-Ig)

    IHC staining of human liver using 66779-1-Ig (same clone as 66779-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66779-1-Ig (CD16 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66779-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using CD16 Monoclonal antibody (66779-1-Ig)

    IHC staining of human liver using 66779-1-Ig (same clone as 66779-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66779-1-Ig (CD16 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66779-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD16 Monoclonal antibody (66779-1-Ig)

    IHC staining of human tonsillitis using 66779-1-Ig (same clone as 66779-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66779-1-Ig (CD16 antibody) at dilution of 1:6000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66779-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CD16 Monoclonal antibody (66779-1-Ig)

    IF Staining of human tonsillitis using 66779-1-Ig (same clone as 66779-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using CD16 antibody (66779-1-Ig, Clone: 1E12E11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66779-1-PBS in a different storage buffer formulation.

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CD16 Monoclonal antibody, PBS Only

CD16 Monoclonal Antibody for IHC, IF-P, ELISA
Cat No. 66779-1-PBS
Clone No.1E12E11

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IHC, IF-P, ELISA

Synonyms

Fc gamma RIII alpha, Fc gamma RIII, CD16-II, CD16a antigen, CD16A

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66779-1-PBS targets CD16 in IHC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD16 fusion protein Ag9787 相同性解析による交差性が予測される生物種
Full Name Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
Calculated molecular weight 254 aa, 29 kDa
GenBank accession numberBC017865
Gene Symbol CD16a
Gene ID (NCBI) 2214
ENSEMBL Gene IDENSG00000203747
RRIDAB_2918477
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP08637
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD16 is a 50-70-kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD16 has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and the FCGR3B.