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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD16 Recombinant antibody

CD16 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

230337G12

Cat no : 83110-1-RR

Synonyms

Fc gamma RIII alpha, Fc gamma RIII, CD16-II, CD16a antigen, CD16A

Validation Data Gallery

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  • Western Blot (WB) analysis of human placenta tissue using CD16 Recombinant antibody (83110-1-RR)

    WB analysis of human placenta using 83110-1-RR

    human placenta tissue was subjected to SDS PAGE followed by western blot with 83110-1-RR (CD16 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83110-1-RR

    Affinity and Kinetic Characterization of 83110-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83110-1-RR against Human CD16 were performed. The affinity constant is 71 nM.

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Tested Applications

Positive WB detected inhuman placenta tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83110-1-RR targets CD16 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
Calculated molecular weight 254 aa, 29 kDa
Observed molecular weight55 kDa
GenBank accession numberBC017865
Gene symbol CD16a
Gene ID (NCBI) 2214
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD16 is a 50-70-kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD16 has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and FCGR3B.

Protocols

Product Specific Protocols
WB protocol for CD16 antibody 83110-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols