FITC Anti-Human CD161 (HP-3G10)
CD161 Monoclonal Antibody for FC
Host / Isotype
Mouse / IgG1, kappa
Reactivity
Human
Applications
FC
Conjugate
FITC Fluorescent Dye
CloneNo.
HP-3G10
Cat no : FITC-65115
Synonyms
Validation Data Gallery
Tested Applications
| Positive FC detected in | human peripheral blood lymphocytes |
Recommended dilution
| Application | Dilution |
|---|---|
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
FITC-65115 targets CD161 in FC applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Human NK cells 相同性解析による交差性が予測される生物種 |
| Full Name | killer cell lectin-like receptor subfamily B, member 1 |
| Calculated molecular weight | 225 aa, 25 kDa |
| GenBank accession number | BC114516 |
| Gene symbol | CD161 |
| Gene ID (NCBI) | 3820 |
| RRID | AB_2883785 |
| Conjugate | FITC Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 498 nm / 526 nm |
| Form | Liquid |
| Purification Method | Affinity purification |
| Storage Buffer | Phosphate based buffer with 0.09% sodium azide and 0.1% gelatin, pH 7.2. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
CD161, also known as KLRB1 or NKR-P1A, is a type II transmembrane C-type lectin-like receptor and is expressed on the cell membrane as disulfide-linked homodimer (PMID: 8077657). It is expressed by the majority of NK cells and subsets of peripheral T cells, including both CD4+ and CD8+ T cells, and is expressed preferentially on adult T cells with a "memory" antigenic phenotype (PMID: 8077657; 22566826). Expression of CD161 correlates with the cytotoxic function of CD16+ NK cells, and ligation of CD161 with its ligand LLT1 inhibits NK cell cytotoxicity and cytokine secretion (PMID: 29686665).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for FITC CD161 antibody FITC-65115 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
