- Featured Product
- KD/KO Validated
CD163 Polyclonal antibody
CD163 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, pig and More (1)
Applications
WB, IHC, IF-P, IP, ELISA
Conjugate
Unconjugated
173
Cat no : 16646-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | pig spleen tissue, human placenta tissue |
Positive IP detected in | human placenta tissue |
Positive IHC detected in | human tonsillitis tissue, human liver tissue, human placenta tissue, human lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | human tonsillitis tissue, human liver tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 1 publications below |
WB | See 32 publications below |
IHC | See 72 publications below |
IF | See 88 publications below |
FC | See 5 publications below |
Product Information
16646-1-AP targets CD163 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, pig samples.
Tested Reactivity | human, mouse, pig |
Cited Reactivity | human, mouse, rat, pig |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | CD163 fusion protein Ag10029 相同性解析による交差性が予測される生物種 |
Full Name | CD163 molecule |
Calculated molecular weight | 1156 aa, 125 kDa |
Observed molecular weight | 130-150 kDa |
GenBank accession number | BC051281 |
Gene symbol | CD163 |
Gene ID (NCBI) | 9332 |
RRID | AB_2756528 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CD163 is a transmembrane protein which belongs to the scavenger receptor cysteine-rich (SRCR) superfamily. This protein is a scavenger receptor for the hemoglobin-haptoglobin complex and is a marker for monocytes and macrophages. Soluble CD163 (sCD163), as a result of ectodomain shedding during inflammatory activation of macrophages, circulates in blood and has been suggested as a plasma/serum marker for macrophage activity.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for CD163 antibody 16646-1-AP | Download protocol |
IHC protocol for CD163 antibody 16646-1-AP | Download protocol |
IF protocol for CD163 antibody 16646-1-AP | Download protocol |
IP protocol for CD163 antibody 16646-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Mil Med Res Caveolin-1 is critical for hepatic iron storage capacity in the development of nonalcoholic fatty liver disease | ||
J Clin Invest CAP2 promotes gastric cancer metastasis by mediating the interaction between tumor cells and tumor-associated macrophages | ||
Adv Sci (Weinh) Inflammatory Fibroblast-Like Synoviocyte-Derived Exosomes Aggravate Osteoarthritis via Enhancing Macrophage Glycolysis | ||
Cell Rep Med Targeting neoadjuvant chemotherapy-induced metabolic reprogramming in pancreatic cancer promotes anti-tumor immunity and chemo-response | ||
Nat Commun Single-cell analysis of human glioma and immune cells identifies S100A4 as an immunotherapy target. | ||
Redox Biol Lactate-induced activation of tumor-associated fibroblasts and IL-8-mediated macrophage recruitment promote lung cancer progression |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Jaouhara (Verified Customer) (12-05-2023) | Works very well in immunohistochemistry (human cartilage)
|
FH Kenzo (Verified Customer) (08-30-2023) | Works well by IF on frozen mouse liver tissue sections.
|
FH Yasuyo (Verified Customer) (05-27-2022) | Works pretty good.
|
FH Emma (Verified Customer) (11-29-2021) | Works well by IF on FFPE tissue @ 1:100 with a Tris-EDTA antigen retrieval.
|