Validation Data Gallery

Filter: AllFC

FC experiment of human PBMCs using 65501-1-RR (same clone as 65501-1-PBS)
1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD163 Rabbit Recombinant Antibody (65501-1-RR, Clone:GHI/61) or Rabbit IgG control PolyAb and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were then stained with CL488-Conjugated CD14. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. Monocytes were gated. This data was developed using the same antibody clone with 65501-1-PBS in a different storage buffer formulation.
×
1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD163 Rabbit Recombinant Antibody (65501-1-RR, Clone:GHI/61) or Rabbit IgG control PolyAb and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were then stained with CL488-Conjugated CD14. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. Monocytes were gated. This data was developed using the same antibody clone with 65501-1-PBS in a different storage buffer formulation.
FC experiment of human PBMCs using 65501-1-PBS
1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD163 Rabbit Recombinant Antibody (65501-1-PBS, Clone:GHI/61) or Rabbit IgG control PolyAb and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were then stained with CL488-Conjugated CD14. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. Monocytes were gated.
×
1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD163 Rabbit Recombinant Antibody (65501-1-PBS, Clone:GHI/61) or Rabbit IgG control PolyAb and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were then stained with CL488-Conjugated CD14. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. Monocytes were gated.
FC experiment of human PBMCs using 65501-1-PBS
1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD163 Rabbit Recombinant Antibody (65501-1-PBS, Clone:GHI/61) or Rabbit IgG control PolyAb and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. Monocytes were gated.
FC experiment of human PBMCs using 65501-1-RR (same clone as 65501-1-PBS)
1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD163 Rabbit Recombinant Antibody (65501-1-RR, Clone:GHI/61) or Rabbit IgG control PolyAb and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. Monocytes were gated. This data was developed using the same antibody clone with 65501-1-PBS in a different storage buffer formulation.
×
1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD163 Rabbit Recombinant Antibody (65501-1-RR, Clone:GHI/61) or Rabbit IgG control PolyAb and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. Monocytes were gated. This data was developed using the same antibody clone with 65501-1-PBS in a different storage buffer formulation.
FC experiment of human PBMCs using 65501-1-RR (same clone as 65501-1-PBS)
1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD163 Rabbit Recombinant Antibody (65501-1-RR, Clone:GHI/61) (left) and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 or 0.2 ug Anti-Human CD163 Mouse Monoclonal Antibody (Clone:GHI/61) (center and right) and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were then stained with CL488-Conjugated CD14. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. Monocytes were gated. This data was developed using the same antibody clone with 65501-1-PBS in a different storage buffer formulation.
×
1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD163 Rabbit Recombinant Antibody (65501-1-RR, Clone:GHI/61) (left) and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 or 0.2 ug Anti-Human CD163 Mouse Monoclonal Antibody (Clone:GHI/61) (center and right) and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were then stained with CL488-Conjugated CD14. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. Monocytes were gated. This data was developed using the same antibody clone with 65501-1-PBS in a different storage buffer formulation.
FC experiment of human PBMCs using 65501-1-PBS
1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD163 Rabbit Recombinant Antibody (65501-1-PBS, Clone:GHI/61) (left) and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 or 0.2 ug Anti-Human CD163 Mouse Monoclonal Antibody (Clone:GHI/61) (center and right) and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were then stained with CL488-Conjugated CD14. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. Monocytes were gated.
×
1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD163 Rabbit Recombinant Antibody (65501-1-PBS, Clone:GHI/61) (left) and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 or 0.2 ug Anti-Human CD163 Mouse Monoclonal Antibody (Clone:GHI/61) (center and right) and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were then stained with CL488-Conjugated CD14. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. Monocytes were gated.

Anti-Human CD163 (GHI/61) Rabbit IgG Recombinant Antibody, PBS Only

CD163 Recombinant Antibody for FC

Cat No. 65501-1-PBS

Clone No.GHI/61

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Synonyms

Scavenger receptor cysteine-rich type 1 protein M130, MM130, M130, Hemoglobin scavenger receptor, GHI/61

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive FC detected in

human PBMCs

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

65501-1-PBS targets CD163 in FC applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	Glycoprotein preparation from hairy cell leukemia spleen 相同性解析による交差性が予測される生物種
Full Name	CD163 molecule
Calculated molecular weight	1156 aa, 125 kDa
GenBank accession number	BC051281
Gene Symbol	CD163
Gene ID (NCBI)	9332
ENSEMBL Gene ID	ENSG00000177575
Conjugate	Unconjugated
Form	Liquid
Purification Method	Affinity purification
Storage Buffer	PBS only
Storage Conditions	Store at -80°C. Stable for one year after shipment.

Background Information

CD163, also known as M130, is a membrane glycoprotein that belongs to the scavenger receptor superfamily (PMID: 8370408). It is an acute phase-regulated and signal-inducing macrophage protein expressed exclusively in monocytes and tissue macrophages (PMID: 11196644). CD163 mediates endocytosis of haptoglobin-haemoglobin complexes (PMID: 11196644). The uptake of haptoglobin by macrophages contributes to the recycling of iron and also to the inflammatory response (PMID: 22900885). Soluble CD163 (sCD163), as a result of ectodomain shedding during inflammatory activation of macrophages, circulates in blood and has been suggested as a plasma/serum marker for macrophage activity (PMID: 12570164).

Protocols

Product Specific Protocols
FC protocol for CD163 antibody 65501-1-PBS	Download protocol

Standard Protocols
Click here to view our Standard Protocols

製品

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カスタム対応

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研究分野

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

FC experiment of human PBMCs using 65501-1-RR (same clone as 65501-1-PBS)

FC experiment of human PBMCs using 65501-1-PBS

FC experiment of human PBMCs using 65501-1-PBS

FC experiment of human PBMCs using 65501-1-RR (same clone as 65501-1-PBS)

FC experiment of human PBMCs using 65501-1-RR (same clone as 65501-1-PBS)

FC experiment of human PBMCs using 65501-1-PBS

Anti-Human CD163 (GHI/61) Rabbit IgG Recombinant Antibody, PBS Only

CD163 Recombinant Antibody for FC

-/ -

Tested Applications

Recommended dilution

Product Information

Background Information

Protocols