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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human CD163 (GHI/61) Rabbit Recombinant Antibody, PBS only

CD163 Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

FC

Conjugate

Unconjugated

CloneNo.

GHI/61

Cat no : 65501-1-PBS

Synonyms

CD163, CD163 molecule, Hemoglobin scavenger receptor, M130, MM130, sCD163

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD163 (GHI/61) (65501-1-PBS)

    FC experiment of human PBMCs using 65501-1-PBS

    1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD163 Rabbit Recombinant Antibody (65501-1-PBS, Clone:GHI/61) or Rabbit IgG control PolyAb and CoraLite®647-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were then stained with CL488-Conjugated CD14. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. Monocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD163 (GHI/61) (65501-1-PBS)

    FC experiment of human PBMCs using 65501-1-PBS

    1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD163 Rabbit Recombinant Antibody (65501-1-PBS, Clone:GHI/61) or Rabbit IgG control PolyAb and CoraLite®647-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. Monocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD163 (GHI/61) (65501-1-PBS)

    FC experiment of human PBMCs using 65501-1-PBS

    1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD163 Rabbit Recombinant Antibody (65501-1-PBS, Clone:GHI/61) (left) and CoraLite®647-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 or 0.2 ug Anti-Human CD163 Mouse Monoclonal Antibody (Clone:GHI/61) (center and right) and CoraLite®647-Conjugated Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were then stained with CL488-Conjugated CD14. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. Monocytes were gated.

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Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

65501-1-PBS targets CD163 in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Glycoprotein preparation from hairy cell leukemia spleen 相同性解析による交差性が予測される生物種
Full Name CD163 molecule
Calculated molecular weight 1156 aa, 125 kDa
GenBank accession numberBC051281
Gene symbol CD163
Gene ID (NCBI) 9332
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C. Stable for one year after shipment.

Background Information

CD163, also known as M130, is a membrane glycoprotein that belongs to the scavenger receptor superfamily (PMID: 8370408). It is an acute phase-regulated and signal-inducing macrophage protein expressed exclusively in monocytes and tissue macrophages (PMID: 11196644). CD163 mediates endocytosis of haptoglobin-haemoglobin complexes (PMID: 11196644). The uptake of haptoglobin by macrophages contributes to the recycling of iron and also to the inflammatory response (PMID: 22900885). Soluble CD163 (sCD163), as a result of ectodomain shedding during inflammatory activation of macrophages, circulates in blood and has been suggested as a plasma/serum marker for macrophage activity (PMID: 12570164).

Protocols

Product Specific Protocols
FC protocol for CD163 antibody 65501-1-PBSDownload protocol
Standard Protocols
Click here to view our Standard Protocols