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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Mouse CD163 Recombinant antibody, PBS Only (Capture/Detector)

CD163 Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240233G5

Cat no : 83285-2-PBS

Synonyms

sCD163, M130, 240233G5

Validation Data Gallery

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  • Sandwich ELISA standard curve of MP00326-3

    Sandwich ELISA standard curve of MP00326-3

    Sandwich ELISA standard curve of MP00326-3, mouse CD163 Recombinant Matched Antibody Pair - PBS only. 83285-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard: Eg0794. 83285-2-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL..

  • Sample test of MP00326-3

    Sample test of MP00326-3

    For the mouse lung supernatant cultured for 3 days, the mean mouse CD163 concentration was determined to be 154.4 pg/mL in ConA stimulated mouse lung supernatant.

  • Sample test of MP00326-3

    Sample test of MP00326-3

    Serum of sixteen mice was measured. The CD163 concentration of detected samples was determined to be 92.2 ng/mL with a range of 46.2-146.5 ng/mL.

  • Cytometric bead array standard curve of MP00326-1

    Cytometric bead array standard curve of MP00326-1

    Cytometric bead array standard curve of MP00326-1, MOUSE CD163 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83285-2-PBS. Detection antibody: 83285-1-PBS. Standard: Eg0794. Range: 0.313-40ng/mL.

  • Cytometric bead array standard curve of MP00326-2

    Cytometric bead array standard curve of MP00326-2

    Cytometric bead array standard curve of MP00326-2, MOUSE CD163 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83285-2-PBS. Detection antibody: 83285-3-PBS. Standard: Eg0794. Range: 0.313-40ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83285-2-PBS targets CD163 as part of a matched antibody pair:

MP00326-1: 83285-2-PBS capture and 83285-1-PBS detection (validated in Cytometric bead array)

MP00326-2: 83285-2-PBS capture and 83285-3-PBS detection (validated in Cytometric bead array)

MP00326-3: 83285-1-PBS capture and 83285-2-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name CD163 antigen
Calculated molecular weight121 kDa
GenBank accession numberNM_053094.2
Gene symbol Cd163
Gene ID (NCBI) 93671
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD163, also known as M130, is a membrane glycoprotein that belongs to the scavenger receptor superfamily (PMID: 8370408). It is an acute phase-regulated and signal-inducing macrophage protein expressed exclusively in monocytes and tissue macrophages (PMID: 11196644). CD163 mediates endocytosis of haptoglobin-haemoglobin complexes (PMID: 11196644). The uptake of haptoglobin by macrophages contributes to the recycling of iron and also to the inflammatory response (PMID: 22900885). Soluble CD163 (sCD163), as a result of ectodomain shedding during inflammatory activation of macrophages, circulates in blood and has been suggested as a plasma/serum marker for macrophage activity (PMID: 12570164).