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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Mouse CD163 Recombinant antibody, PBS Only

CD163 Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

240233A3

Cat no : 83285-4-PBS

Synonyms

sCD163, M130, 240233A3

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using CD163 Recombinant antibody (83285-4-RR)

    WB analysis using 83285-4-RR (same clone as 83285-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83285-4-RR (CD163 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83285-4-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat spleen tissue using CD163 Recombinant antibody (83285-4-RR)

    WB analysis of rat spleen using 83285-4-RR (same clone as 83285-4-PBS)

    rat spleen tissue were subjected to SDS PAGE followed by western blot with 83285-4-RR (CD163 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83285-4-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse lung tissue using CD163 Recombinant antibody (83285-4-RR)

    WB analysis of mouse lung using 83285-4-RR (same clone as 83285-4-PBS)

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 83285-4-RR (CD163 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83285-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using CD163 Recombinant antibody (83285-4-RR)

    IHC staining of mouse liver using 83285-4-RR (same clone as 83285-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83285-4-RR (CD163 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83285-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using CD163 Recombinant antibody (83285-4-RR)

    IHC staining of mouse liver using 83285-4-RR (same clone as 83285-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83285-4-RR (CD163 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83285-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using CD163 Recombinant antibody (83285-4-RR)

    IHC staining of mouse spleen using 83285-4-RR (same clone as 83285-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83285-4-RR (CD163 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83285-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using CD163 Recombinant antibody (83285-4-RR)

    IHC staining of mouse spleen using 83285-4-RR (same clone as 83285-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83285-4-RR (CD163 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83285-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83285-4-RR

    Affinity and Kinetic Characterization of 83285-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83285-4-RR against Mouse CD163 were performed. The affinity constant is 2.25 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83285-4-PBS targets CD163 in WB, IHC, ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name CD163 antigen
Calculated molecular weight121 kDa
GenBank accession numberNM_053094.2
Gene symbol Cd163
Gene ID (NCBI) 93671
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.