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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD164 Polyclonal antibody

CD164 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12083-2-AP

Synonyms

CD164, CD164 molecule, sialomucin, endolyn, MUC 24, Sialomucin core protein 24

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD164 Polyclonal antibody (12083-2-AP)

    WB analysis using 12083-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12083-2-AP (CD164 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using CD164 Polyclonal antibody (12083-2-AP)

    IHC staining of human pancreas cancer using 12083-2-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12083-2-AP (CD164 antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

Positive WB detected inBxPC-3 cells, U-87 MG cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12083-2-AP targets CD164 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD164 fusion protein Ag2715 相同性解析による交差性が予測される生物種
Full Name CD164 molecule, sialomucin
Calculated molecular weight 197 aa, 21 kDa
Observed molecular weight 82 kDa
GenBank accession numberBC011522
Gene symbol CD164
Gene ID (NCBI) 8763
RRIDAB_2072583
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Sialomucins are a heterogeneous group of secreted or membrane-associated mucins that appear to play 2 key but opposing roles in vivo: first as cytoprotective or antiadhesive agents, and second as adhesion receptors. CD164 is a type I integral transmembrane sialomucin that functions as an adhesion receptor (PMID: 9680353)(PMID: 17077324). Sialomucin CD164 (MUC-24), also referred to multi-glycosylated core protein 24 (MGC24), is known to function as a receptor that regulates stem cell localization to the bone marrow. CD164 may play a key role in hematopoiesis by facilitating the adhesion of CD34+ cells to the stroma and by negatively regulating CD34+CD38(lo/-) cell proliferation. Important role of CD164 in in prostate cancer metastasis, promoting myogenesis and regulating myoblast migration so far have been revealed (PMID: 9763543)(PMID: 16859559)(PMID: 17077324).

Protocols

Product Specific Protocols
WB protocol for CD164 antibody 12083-2-APDownload protocol
IHC protocol for CD164 antibody 12083-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols