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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human CD16b Rabbit Recombinant Antibody, PBS Only

CD16b Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

241713E3

Cat no : 98192-1-PBS

Synonyms

FCGR3B, FCG3, Fc gamma RIIIb, Fc gamma RIII beta, Fc gamma RIII

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human peripheral blood leukocytes using Anti-Human CD16b Rabbit Recombinant Antibody (98192-1-RR)

    FC experiment of human peripheral blood leukocytes using 98192-1-RR (same clone as 98192-1-PBS)

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD16b Rabbit Recombinant Antibody (98192-1-RR, Clone:241713E3) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed. This data was developed using the same antibody clone with 98192-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of human peripheral blood leukocytes using Anti-Human CD16b Rabbit Recombinant Antibody (98192-1-RR)

    FC experiment of human peripheral blood leukocytes using 98192-1-RR (same clone as 98192-1-PBS)

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD16b Rabbit Recombinant Antibody (98192-1-RR, Clone:241713E3) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed. This data was developed using the same antibody clone with 98192-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 98192-1-PBS is CD16b Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name Fc fragment of IgG, low affinity IIIb, receptor (CD16b)
Gene symbol FCGR3B
Gene ID (NCBI) 2215
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD16 is a low affinity Fc receptor. It has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and FCGR3B. In humans, FcγRIIIa/CD16a is a type I transmembrane receptor expressed on macrophages and NK cells. FcγRIIIb/CD16b is a GPI-linked Fc-receptor highly expressed on neutrophils. The two proteins differ by only four amino acid residues in the mature proteins (PMID: 30361439).