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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD177 Polyclonal antibody

CD177 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 18264-1-AP

Synonyms

CD177, CD177 antigen, CD177 molecule, HNA 2a, HNA2A, NB1, NB1 glycoprotein, NB1 GP, PRV 1, PRV1

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse bone marrow using CD177 Polyclonal antibody (18264-1-AP)

    WB analysis of mouse bone marrow using 18264-1-AP

    mouse bone marrow tissue was subjected to SDS PAGE followed by western blot with 18264-1-AP (CD177 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inmouse bone marrow

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18264-1-AP targets CD177 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD177 fusion protein Ag13146 相同性解析による交差性が予測される生物種
Full Name CD177 molecule
Calculated molecular weight 437 aa, 46 kDa
Observed molecular weight100-130 kDa
GenBank accession numberBC029167
Gene symbol CD177
Gene ID (NCBI) 57126
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD177, also known as NB1, HNA-2a or PRV-1, is a glycosylphosphatidylinositol (GPI)-anchored glycoprotein that belongs to the uPAR/CD59/Ly6 snake toxin superfamily (PMID: 17898576). It is expressed exclusively by neutrophils, neutrophilic metamyelocytes, and myelocytes (PMID: 17580308). CD177 interacts with β2 integrin CD11b/CD18 and CD11a/CD18, PECAM-1, and participates in neutrophil activation and transmigration (PMID: 17580308; 21193407). CD177 has been identified as a useful biomarker for myeloproliferative diseases (PMID: 17898576).

Protocols

Product Specific Protocols
WB protocol for CD177 antibody 18264-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols