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Validation Data Gallery

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  • Flow cytometry (FC) experiment of Transfected HEK-293T cells using Anti-Human CD178 (Fas ligand) (NOK-1) Mouse IgG2a (65598-1-MR)

    FC experiment of Transfected HEK-293T using 65598-1-MR

    1x10^6 HEK-293T cells or CD178 transfected HEK-293T cells were surface stained with 0.25 ug Anti-Human CD178 (Fas ligand) (NOK-1) Mouse IgG2a RecAb (65598-1-MR, Clone: NOK-1) and PE-conjugated Goat Anti-Mouse IgG. Cells were not fixed.

  • Flow cytometry (FC) experiment of Transfected HEK-293T cells using Anti-Human CD178 (Fas ligand) (NOK-1) Mouse IgG2a (65598-1-MR)

    FC experiment of Transfected HEK-293T using 65598-1-MR

    1x10^6 HEK-293T cells or CD178 transfected HEK-293T cells were surface stained with 0.25 ug Anti-Human CD178 (Fas ligand) (NOK-1) Mouse IgG2a RecAb (65598-1-MR, Clone: NOK-1) and PE-conjugated Goat Anti-Mouse IgG (red), or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were not fixed.

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Anti-Human CD178 (Fas ligand) (NOK-1) Mouse IgG2a Recombinant Antibody

CD178 (Fas ligand) Uni-rAbTM Recombinant Antibody for FC
Cat No. 65598-1-MR
Clone No.NOK-1

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Synonyms

CD178, Fas ligand, FASLG, CD95-L, CD95L

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive FC detected inTransfected HEK-293T cells

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65598-1-MR targets CD178 (Fas ligand) in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Immunogen L5178Y mouse T lymphoma cells expressing recombinant human FasL 相同性解析による交差性が予測される生物種
Full Name Fas ligand (TNF superfamily, member 6)
Calculated molecular weight 281 aa, 31 kDa
GenBank accession numberBC017502
Gene Symbol Fas Ligand
Gene ID (NCBI) 356
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide , pH 7.3
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

CD178, also known as Fas ligand, is a type II transmembrane protein that belongs to the tumor necrosis factor (TNF) family. It is expressed on NK cells, cytotoxic T lymphocytes and activated CD4+ Th1 cells. CD178 transduces apoptotic signal into cells by binding to FAS/CD95. It is involved as a death factor in the regulation of activation-induced cell death, establishment of immune privilege and tumor cell survival.

Protocols

Product Specific Protocols
FC protocol for CD178 (Fas ligand) antibody 65598-1-MRDownload protocol
Standard Protocols
Click here to view our Standard Protocols