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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD19 Polyclonal antibody

CD19 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 27914-1-AP

Synonyms

Differentiation antigen CD19, CD19 molecule, B-lymphocyte surface antigen B4, B-lymphocyte antigen CD19, B4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD19 Polyclonal antibody (27914-1-AP)

    WB analysis using 27914-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27914-1-AP (CD19 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Raji cells using CD19 Polyclonal antibody (27914-1-AP)

    IP experiment of Raji using 27914-1-AP

    IP result of anti-CD19 (IP:27914-1-AP, 4ug; Detection:27914-1-AP 1:3000) with Raji cells lysate 1800 ug.

  • Immunofluorescence (IF) / fluorescent staining of Raji cells using CD19 Polyclonal antibody (27914-1-AP)

    IF Staining of Raji using 27914-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using CD19 antibody (27914-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

Positive WB detected inDaudi cells, Raji cells, Ramos cells
Positive IP detected inRaji cells
Positive IF/ICC detected inRaji cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27914-1-AP targets CD19 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD19 fusion protein Ag27404 相同性解析による交差性が予測される生物種
Full Name CD19 molecule
Calculated molecular weight 556 aa, 61 kDa
Observed molecular weight95-100 kDa
GenBank accession numberBC006338
Gene symbol CD19
Gene ID (NCBI) 930
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD19 is a 95 kDa type I transmembrane glycoprotein belonging to the immunoglobulin superfamily (PMID: 2472450). It is expressed by B cells and follicular dendritic cells. CD19 is up-regulated at the step of B-lineage commitment during the differentiation of the hematopoietic stem cell, it remains on during subsequent stages of differentiation until finally down-regulated during terminal differentiation into plasma cells (PMID: 8528044). CD19 is involved in B cell development, activation and differentiation. It is the dominant component for the signaling complex on B cells that includes CD21 (CR2), CD81 (TAPA-1) and CD225 and acts as a critical co-receptor for BCR signal transduction (PMID: 23210908).

Protocols

Product Specific Protocols
WB protocol for CD19 antibody 27914-1-APDownload protocol
IF protocol for CD19 antibody 27914-1-APDownload protocol
IP protocol for CD19 antibody 27914-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols