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CD19 Polyclonal antibody

Name: CD19 Polyclonal antibody
Brand: Proteintech
SKU: 27949-1-AP

CD19 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Rat, Mouse and More (3)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 27949-1-AP

Synonyms

B lymphocyte antigen CD19, B4, CD19, CD19 molecule, Differentiation antigen CD19, T cell surface antigen Leu 12

Validation Data Gallery

Filter:

AllWBIHCIF/ICC

WB analysis of Raji using 27949-1-AP
WB result of CD19 antibody (27949-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CD19 transfected Raji cells.
WB analysis using 27949-1-AP
Daudi cells were subjected to SDS PAGE followed by western blot with 27949-1-AP (CD19 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis using 27949-1-AP
Raji cell (CD19 positive) and Jurkat (CD19 negative) were subjected to SDS PAGE followed by western blot with 27949-1-AP (CD19 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IHC staining of human tonsillitis using 27949-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27949-1-AP (CD19 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 27949-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27949-1-AP (CD19 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse colon using 27949-1-AP
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 27949-1-AP (CD19 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse colon using 27949-1-AP
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 27949-1-AP (CD19 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lymphoma using 27949-1-AP
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 27949-1-AP (CD19 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lymphoma using 27949-1-AP
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 27949-1-AP (CD19 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat colon using 27949-1-AP
Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 27949-1-AP (CD19 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat thymus using 27949-1-AP
Immunohistochemical analysis of paraffin-embedded rat thymus tissue slide using 27949-1-AP (CD19 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat thymus using 27949-1-AP
Immunohistochemical analysis of paraffin-embedded rat thymus tissue slide using 27949-1-AP (CD19 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of Raji using 27949-1-AP
Immunofluorescent analysis of (4% PFA) fixed Raji cells using CD19 antibody (27949-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 27949-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	Daudi cells, Raji cells
Positive IHC detected in	human tonsillitis tissue, mouse colon tissue, human lymphoma tissue, rat colon tissue, rat thymus tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	Raji cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunohistochemistry (IHC)	IHC : 1:50-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 1 publications below
IHC	See 7 publications below
IF	See 2 publications below

Product Information

27949-1-AP targets CD19 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Rat, Mouse samples.

Tested Reactivity	Human, Rat, Mouse
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CD19 fusion protein Ag27656 相同性解析による交差性が予測される生物種
Full Name	CD19 molecule
Calculated molecular weight	556 aa, 61 kDa
Observed molecular weight	95 kDa
GenBank accession number	BC006338
Gene symbol	CD19
Gene ID (NCBI)	930
RRID	AB_2881019
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CD19 is a B cell-specific molecule that controls B cell activation by complexing with the BCR. CD19 is a member of the Ig superfamily and is the dominant component for the signaling complex on B cells that includes CD21, CD81, and CD22. Humans with CD19 deficiency have reduced proliferative responses to BCR stimulation in vitro and mount impaired Ab responses to vaccination.

Protocols

Product Specific Protocols
WB protocol for CD19 antibody 27949-1-AP	Download protocol
IHC protocol for CD19 antibody 27949-1-AP	Download protocol
IF protocol for CD19 antibody 27949-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
rat	IHC	J Adv Res Zearalenone-14-glucoside specifically promotes dysplasia of Gut-Associated Lymphoid Tissue: A natural product for constructing intestinal nodular lymphatic hyperplasia model Authors - Haonan Ruan View Article
human	IHC	Int J Mol Sci CXCR4 Expressed by Tumor-Infiltrating B Cells in Gastric Cancer Related to Survival in the Tumor Microenvironment: An Analysis Combining Single-Cell RNA Sequencing with Bulk RNA Sequencing Authors - Chen Su View Article
human	WB	Int J Biol Macromol Exosome co-delivery of a STING agonist augments immunogenicity elicited by CVB3 VP1 vaccine via promoting antigen cross-presentation of CD8 + DCs Authors - Changwei Zhang View Article
human	IF	bioRxiv A Novel Humanized Mouse Model for HIV and Tuberculosis Co-infection Studies Authors - José Alejandro Bohórquez View Article
human	IHC	Cancer Lett Comprehensive molecular characterization of long-term glioblastoma survivors Authors - Hao Xu View Article
	IHC	J Ethnopharmacol Effective substances and molecular mechanisms guided by network pharmacology: An example study of Scrophulariae Radix treatment of hyperthyroidism and thyroid hormone-induced liver and kidney injuries Authors - Meng-Yuan Sheng View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD19 Polyclonal antibody

CD19 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Validation Data Gallery

WB analysis of Raji using 27949-1-AP

WB analysis using 27949-1-AP

WB analysis using 27949-1-AP

IHC staining of human tonsillitis using 27949-1-AP

IHC staining of human tonsillitis using 27949-1-AP

IHC staining of mouse colon using 27949-1-AP

IHC staining of mouse colon using 27949-1-AP

IHC staining of human lymphoma using 27949-1-AP

IHC staining of human lymphoma using 27949-1-AP

IHC staining of rat colon using 27949-1-AP

IHC staining of rat thymus using 27949-1-AP

IHC staining of rat thymus using 27949-1-AP

IF Staining of Raji using 27949-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Zearalenone-14-glucoside specifically promotes dysplasia of Gut-Associated Lymphoid Tissue: A natural product for constructing intestinal nodular lymphatic hyperplasia model

CXCR4 Expressed by Tumor-Infiltrating B Cells in Gastric Cancer Related to Survival in the Tumor Microenvironment: An Analysis Combining Single-Cell RNA Sequencing with Bulk RNA Sequencing

Exosome co-delivery of a STING agonist augments immunogenicity elicited by CVB3 VP1 vaccine via promoting antigen cross-presentation of CD8 + DCs

A Novel Humanized Mouse Model for HIV and Tuberculosis Co-infection Studies

Comprehensive molecular characterization of long-term glioblastoma survivors

Effective substances and molecular mechanisms guided by network pharmacology: An example study of Scrophulariae Radix treatment of hyperthyroidism and thyroid hormone-induced liver and kidney injuries