• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Mouse CD19 (1D3)

CD19 Monoclonal Antibody for IF-Fro, FC

Host / Isotype

Rat / IgG2a, kappa

Reactivity

mouse

Applications

IF-Fro, FC

Conjugate

Unconjugated

CloneNo.

1D3

Cat no : 65290-1-Ig

Synonyms

CD19 antigen, 1D3

Validation Data Gallery

Filter:
  • Immunofluorescence (IF) / fluorescent staining of mouse spleen tissue using Anti-Mouse CD19 (1D3) (65290-1-Ig)

    IF Staining of mouse spleen using 65290-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse spleen tissue using CD19 antibody (65290-1-Ig, Clone: 1D3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of C57BL/6 mouse splenocytes using Anti-Mouse CD19 (1D3) (65290-1-Ig)

    FC experiment of C57BL/6 mouse splenocytes using 65290-1-Ig

    1X10^6 C57BL/6 mouse splenocytes were surface co-stained with CoraLite® Plus 647 Anti-Mouse CD3 (17A2) and 0.5 ug Anti-Mouse CD19 (65290-1-Ig, Clone:1D3) or 0.5 ug Rat IgG2a Isotype Control (2A3), and FITC anti-rat IgG2a Antibody at dilution 1:100. Cells were not fixed.

☆ 2/28（金）まで！最大30%オフ！ >> トライアルサイズもお得な一次抗体キャンペーン

Tested Applications

Positive IF-Fro detected inmouse spleen tissue
Positive FC detected inmouse splenocytes

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-FROIF-FRO : 1:200-1:800
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65290-1-Ig targets CD19 in IF-Fro, FC applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rat / IgG2a, kappa
Class Monoclonal
Type Antibody
Immunogen Mouse CD19 Transfected Cell Line 相同性解析による交差性が予測される生物種
Full Name CD19 antigen
GenBank accession numberBC156767
Gene symbol Cd19
Gene ID (NCBI) 12478
RRIDAB_3084916
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

CD19 is a 95 kDa type I transmembrane glycoprotein belonging to the immunoglobulin superfamily (PMID: 2472450). It is expressed by B cells and follicular dendritic cells. CD19 is up-regulated at the step of B-lineage commitment during the differentiation of the hematopoietic stem cell, it remains on during subsequent stages of differentiation until finally down-regulated during terminal differentiation into plasma cells (PMID: 8528044). CD19 is involved in B cell development, activation and differentiation. It is the dominant component for the signaling complex on B cells that includes CD21 (CR2), CD81 (TAPA-1) and CD225 and acts as a critical co-receptor for BCR signal transduction (PMID: 23210908).

Protocols

Product Specific Protocols
IF protocol for CD19 antibody 65290-1-IgDownload protocol
FC protocol for CD19 antibody 65290-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols