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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse spleen tissue using Anti-Mouse CD19 (1D3) (65290-1-Ig)

    IF Staining of mouse spleen using 65290-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse spleen tissue using CD19 antibody (65290-1-Ig, Clone: 1D3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of C57BL/6 mouse splenocytes using Anti-Mouse CD19 (1D3) (65290-1-Ig)

    FC experiment of C57BL/6 mouse splenocytes using 65290-1-Ig

    1X10^6 C57BL/6 mouse splenocytes were surface co-stained with CoraLite® Plus 647 Anti-Mouse CD3 (17A2) and 0.5 ug Anti-Mouse CD19 (65290-1-Ig, Clone:1D3) or 0.5 ug Rat IgG2a Isotype Control (2A3), and FITC anti-rat IgG2a Antibody at dilution 1:100. Cells were not fixed.

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Anti-Mouse CD19 (1D3)

CD19 Monoclonal Antibody for IF-Fro, FC
Cat No. 65290-1-Ig
Clone No.1D3

Host / Isotype

Rat / IgG2a, kappa

Reactivity

mouse

Applications

IF-Fro, FC

Synonyms

CD19 antigen, 1D3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF-Fro detected inmouse spleen tissue
Positive FC detected inmouse splenocytes

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-FROIF-FRO : 1:200-1:800
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65290-1-Ig targets CD19 in IF-Fro, FC applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rat / IgG2a, kappa
Class Monoclonal
Type Antibody
Immunogen Mouse CD19 Transfected Cell Line 相同性解析による交差性が予測される生物種
Full Name CD19 antigen
GenBank accession numberBC156767
Gene Symbol Cd19
Gene ID (NCBI) 12478
RRIDAB_3084916
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

CD19 is a 95 kDa type I transmembrane glycoprotein belonging to the immunoglobulin superfamily (PMID: 2472450). It is expressed by B cells and follicular dendritic cells. CD19 is up-regulated at the step of B-lineage commitment during the differentiation of the hematopoietic stem cell, it remains on during subsequent stages of differentiation until finally down-regulated during terminal differentiation into plasma cells (PMID: 8528044). CD19 is involved in B cell development, activation and differentiation. It is the dominant component for the signaling complex on B cells that includes CD21 (CR2), CD81 (TAPA-1) and CD225 and acts as a critical co-receptor for BCR signal transduction (PMID: 23210908).

Protocols

Product Specific Protocols
IF protocol for CD19 antibody 65290-1-IgDownload protocol
FC protocol for CD19 antibody 65290-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols