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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Mouse CD19 (1D3)

CD19 Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2a, kappa

Reactivity

mouse

Applications

FC

Conjugate

Unconjugated

CloneNo.

1D3

Cat no : 65290-1-Ig

Synonyms

AW495831, Cd19, CD19 antigen

Validation Data Gallery

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  • Flow cytometry (FC) experiment of C57BL/6 mouse splenocytes using Anti-Mouse CD19 (1D3) (65290-1-Ig)

    FC experiment of C57BL/6 mouse splenocytes using 65290-1-Ig

    1X10^6 C57BL/6 mouse splenocytes were surface co-stained with CoraLite® Plus 647 Anti-Mouse CD3 (17A2) and 0.5 ug Anti-Mouse CD19 (65290-1-Ig, Clone:1D3) or 0.5 ug Rat IgG2a Isotype Control (2A3), and FITC anti-rat IgG2a Antibody at dilution 1:100. Cells were not fixed.

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Tested Applications

Positive FC detected inmouse splenocytes

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65290-1-Ig targets CD19 in FC applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rat / IgG2a, kappa
Class Monoclonal
Type Antibody
Immunogen Mouse CD19 Transfected Cell Line 相同性解析による交差性が予測される生物種
Full Name CD19 antigen
GenBank accession numberBC156767
Gene symbol Cd19
Gene ID (NCBI) 12478
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

CD19 is a 95 kDa type I transmembrane glycoprotein belonging to the immunoglobulin superfamily (PMID: 2472450). It is expressed by B cells and follicular dendritic cells. CD19 is up-regulated at the step of B-lineage commitment during the differentiation of the hematopoietic stem cell, it remains on during subsequent stages of differentiation until finally down-regulated during terminal differentiation into plasma cells (PMID: 8528044). CD19 is involved in B cell development, activation and differentiation. It is the dominant component for the signaling complex on B cells that includes CD21 (CR2), CD81 (TAPA-1) and CD225 and acts as a critical co-receptor for BCR signal transduction (PMID: 23210908).

Protocols

Product Specific Protocols
FC protocol for CD19 antibody 65290-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols